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Investigating the evolution of Drosophila STING-dependent antiviral innate immunity by multispecies comparison of 2′3′-cGAMP responses

2024-03-01Journal article Open access
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dc.contributor.authorHédelin, Léna
dc.contributor.authorThiébaut, Antonin
dc.contributor.authorHuang, Jingxian
dc.contributor.authorLi, Xiaoyan
dc.contributor.authorLemoine, Aurélie
dc.contributor.authorHaas, Gabrielle
dc.contributor.authorMeignin, Carine
dc.contributor.authorCai, Hua
dc.contributor.authorWaterhouse, Robert M.
dc.contributor.authorMartins, Nelson
dc.contributor.authorImler, Jean Luc
dc.date.accessioned2024-04-10T12:52:08Z
dc.date.available2024-04-10T12:52:08Z
dc.date.issued2024-03-01
dc.description.abstractViruses represent a major threat to all animals, which defend themselves through induction of a large set of virus- stimulated genes that collectively control the infection. In vertebrates, these genes include interferons that play a critical role in the amplification of the response to infection. Virus- and interferon-stimulated genes include restriction factors targeting the different steps of the viral replication cycle, in addition to molecules associated with inflammation and adaptive immunity. Predictably, antiviral genes evolve dynamically in response to viral pressure. As a result, each animal has a unique arsenal of antiviral genes. Here, we exploit the capacity to experimentally activate the evolutionarily conserved stimulator of IFN genes (STING) signaling pathway by injection of the cyclic dinucleotide 2′3′-cyclic guanosine monophosphate-adenosine monophosphate into flies to define the repertoire of STING-regulated genes in 10 Drosophila species, spanning 40 million years of evolution. Our data reveal a set of conserved STING-regulated factors, including STING itself, a cGAS-like-receptor, the restriction factor pastel, and the antiviral protein Vago, but also 2 key components of the antiviral RNA interference pathway, Dicer-2, and Argonaute2. In addition, we identify unknown species- or lineage-specific genes that have not been previously associated with resistance to viruses. Our data provide insight into the core antiviral response in Drosophila flies and pave the way for the characterization of previously unknown antiviral effectors.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1093/molbev/msae032pt_PT
dc.identifier.eid85188794970
dc.identifier.issn0737-4038
dc.identifier.pmcPMC10917227
dc.identifier.pmid38377349
dc.identifier.urihttp://hdl.handle.net/10400.14/44513
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subject2’3’-cGAMPpt_PT
dc.subjectcGAS/STINGpt_PT
dc.subjectDrosophilapt_PT
dc.subjectEvo-immunopt_PT
dc.subjectTranscriptomept_PT
dc.titleInvestigating the evolution of Drosophila STING-dependent antiviral innate immunity by multispecies comparison of 2′3′-cGAMP responsespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue3pt_PT
oaire.citation.titleMolecular Biology and Evolutionpt_PT
oaire.citation.volume41pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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