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Recovery of heat-injured listeria innocua

2006Journal article Open access
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dc.contributor.authorMiller, Fátima A.
dc.contributor.authorBrandão, Teresa R. S.
dc.contributor.authorTeixeira, Paula
dc.contributor.authorSilva, Cristina L.M.
dc.date.accessioned2010-11-09T15:23:31Z
dc.date.available2010-11-09T15:23:31Z
dc.date.issued2006
dc.description.abstractListeria innocua was subjected to thermal inactivation and the extent of heat-injured cells was quantified. Cultures were heated in liquid medium for different times, using temperatures in the range of 52.5 to 65.0 °C, and plated on Tryptic Soy Agar with 0.6% yeast extract (TSAYE) used as non-selective medium and on TSAYE plus 5% NaCl (TSAYE+NaCl) and Palcam agar with selective supplement (Palcam agar) as selective media. The difference observed in counts in non-selective and in selective media gave an indication of cell injury during the heat treatment. D- and z- values were calculated for all conditions considered. For each temperature, D-values obtained using non-selective recovery procedures were higher than the ones obtained using the two selective media. When comparing the selective media, it can be concluded that Palcam agar allowed recovery and growth of thermally injured cells and so it was less inhibitor than TSAYE+NaCl. Another important result was the influence of temperature on the degree of cellular injury. As temperature increases, the degree of heat-injured cells also increases, and consequently concern has to be taken with the temperature and the counting medium used in food processing studies. The results of this work clearly demonstrated that selective media used for Listeria monocytogenes enumeration/detection might not be suitable for the recovery of heatinjured cells, which can dangerously underestimate the presence of this foodborne pathogen.por
dc.identifier.citationMILLER, Fátima A...[et al.] - Recovery of heat-injured listeria innocua. International Journal of Food Microbiology. ISSN 0168-160. Vol. 112, n.º 3 (2006), p. 261-26por
dc.identifier.urihttp://hdl.handle.net/10400.14/3361
dc.language.isoengpor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7K-4K717MF-1&_user=2460358&_coverDate=12%2F01%2F2006&_rdoc=12&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info%28%23toc%235061%232006%23998879996%23637068%23FLA%23display%23Volume%29&_cdi=5061&_sort=d&_docanchor=&_ct=21&_acct=C000057417&_version=1&_urlVersion=0&_userid=2460358&md5=711f11990b9a64c0eb9143f67df63448&searchtype=apor
dc.subjectListeria innocuapor
dc.subjectThermal inactivationpor
dc.subjectInjurypor
dc.subjectRecoverypor
dc.titleRecovery of heat-injured listeria innocuapor
dc.typejournal article
dspace.entity.typePublication
person.familyNameMiller
person.familyNameBrandão
person.familyNameTeixeira
person.familyNameSilva
person.givenNameFatima
person.givenNameTeresa
person.givenNamePaula
person.givenNameCristina
person.identifierG-5364-2013
person.identifierJ-9418-2012
person.identifier1473674
person.identifier.ciencia-id3A17-0386-6C1B
person.identifier.ciencia-id7B19-E23B-BB31
person.identifier.ciencia-id6611-634A-132C
person.identifier.orcid0000-0002-3715-6231
person.identifier.orcid0000-0002-8857-6471
person.identifier.orcid0000-0002-6296-5137
person.identifier.orcid0000-0002-0495-3955
person.identifier.ridG-5364-2013
person.identifier.ridJ-9418-2012
person.identifier.ridJ-8678-2014
person.identifier.ridA-3568-2013
person.identifier.scopus-author-id15056315100
person.identifier.scopus-author-id6602174128
person.identifier.scopus-author-id7005895206
person.identifier.scopus-author-id7201387397
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscoveryb918d4a2-b9f9-4c56-8318-111625e4d58b

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