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Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water

2019Journal article Open access
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dc.contributor.authorRocha, Jaqueline
dc.contributor.authorFernandes, Telma
dc.contributor.authorRiquelme, Maria V.
dc.contributor.authorZhu, Ni
dc.contributor.authorPruden, Amy
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2019-11-12T19:30:56Z
dc.date.available2019-11-12T19:30:56Z
dc.date.issued2019
dc.description.abstractStandardized methods are needed to support monitoring of antibiotic resistance in environmental samples. Culture-based methods target species of human-health relevance, while the direct quantification of antibiotic resistance genes (ARGs) measures the antibiotic resistance potential in the microbial community. This study compared measurements of tetracycline-, sulphonamide-, and cefotaxime-resistant presumptive total and fecal coliforms and presumptive enterococci versus a suite of ARGs quantified by quantitative polymerase chain reaction (qPCR) across waste-, recycled-, tap-, and freshwater. Cross-laboratory comparison of results involved measurements on samples collected and analysed in the US and Portugal. The same DNA extracts analysed in the US and Portugal produced comparable qPCR results (variation <28%), except for blaOXA-1 gene (0%–57%). Presumptive total and fecal coliforms and cefotaxime-resistant total coliforms strongly correlated with blaCTX-M and intI1 (0.725 ≤ R2 ≤ 0.762; p < 0.0001). Further, presumptive total and fecal coliforms correlated with the Escherichia coli-specific biomarkers, gadAB, and uidA, suggesting that both methods captured fecal-sourced bacteria. The genes encoding resistance to sulphonamides (sul1 and sul2) were the most abundant, followed by genes encoding resistance to tetracyclines (tet(A) and tet(O)) and β-lactams (blaOXA-1 and, blaCTX-M), which was in agreement with the culture-based enumerations. The findings can help inform future application of methods being considered for international antibiotic resistance surveillance in the environment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRocha, J., Fernandes, T., Riquelme, M. V., Zhu, N., Pruden, A., & Manaia, C. M. (2019). Comparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap water. International Journal of Environmental Research and Public Health, 16(21), 4217. https://doi.org/10.3390/ijerph16214217pt_PT
dc.identifier.doi10.3390/ijerph16214217pt_PT
dc.identifier.eid85074343266
dc.identifier.eissn1660-4601
dc.identifier.pmcPMC6862664
dc.identifier.pmid31671709
dc.identifier.urihttp://hdl.handle.net/10400.14/28705
dc.identifier.wos000498842000163
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationOISE 1545756
dc.relationNORTE-08-5369-FSE-000007
dc.relationNORTE-01-0247-FEDER-033330
dc.relation2015/298
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntibiotic resistance monitoringpt_PT
dc.subjectAntibiotic resistant coliformspt_PT
dc.subjectWater qualitypt_PT
dc.titleComparison of culture- and quantitative PCR-Based indicators of antibiotic resistance in wastewater, recycled water, and tap waterpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue21
oaire.citation.titleInternational Journal of Environmental Research and Public Healthpt_PT
oaire.citation.volume16
person.familyNameRocha
person.familyNameFernandes
person.familyNameManaia
person.givenNameJaqueline
person.givenNameTelma
person.givenNameCélia
person.identifier.ciencia-id721C-C3E4-6A38
person.identifier.ciencia-idA41C-5189-7E46
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0003-1651-9483
person.identifier.orcid0000-0002-5954-5609
person.identifier.orcid0000-0002-3674-1789
person.identifier.ridK-3850-2014
person.identifier.scopus-author-id57113035100
person.identifier.scopus-author-id6602465318
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication61cb19ec-ea8b-4294-a77d-378aa7994f16
relation.isAuthorOfPublication71f44b46-f632-4ed5-95e5-11310553c4cd
relation.isAuthorOfPublicationa5f14577-0aed-4046-97da-5f4216c7aa07
relation.isAuthorOfPublication.latestForDiscoverya5f14577-0aed-4046-97da-5f4216c7aa07

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