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Characterization of a bacteriocin of pediococcus pentosaceus SB83 and its potential for vaginal application

2014Journal article Restricted access
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dc.contributor.authorBorges, Sandra
dc.contributor.authorBarbosa, Joana
dc.contributor.authorSilva, Joana
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2015-05-14T11:36:19Z
dc.date.available2015-05-14T11:36:19Z
dc.date.issued2014
dc.description.abstractListeria monocytogenes, the responsible agent for listeriosis, is one of several microorganisms which can be transmitted from mother to fetus/neonate by vertical transmission. The aim of this study was to characterize a bacteriocin produced by Pediococcus pentosaceus SB83 for vaginal application, to prevent colonization of pregnant women with L. monocytogenes. Bacteriocin SB83 showed inhibitory activity against L. monocytogenes (serotypes 1/2a 1/2b and 4b) but did not inhibit vaginal lactic acid bacteria. Additionally, bacteriocin SB83 inhibited the growth of Enterococcus faecalis, Enterococcus faecium and Bacillus subtilis. Bacteriocin SB83 was produced at maximum levels between 24 h and 36 h of growth (6400 AU/mL against serotype 1/2b and 4b, 3200 AU/mL against serotype 1/2a). Significant reduction in antimicrobial activity was observed after treatment of cell-free supernatants with proteinase K, pepsin, papain and trypsin. Bacteriocin SB83 was resistant to treatments with Tween 80, Tween 20, Triton X-100, SDS and EDTA. This bacteriocin was stable at pH values between 4 and 6.5; a decrease in activity was observed at pH 2 and above 8. A decrease in antibacterial activity was recorded at 100 °C and 121° C. In the range 4 °C to 80 °C, antimicrobial activity was not affected. The activity of bacteriocin did not change in simulated vaginal fluid (pH 4.2, 5.5 and 6.5). The molecular size of bacteriocin SB83 was between 3.5 and 6.5 kDa.In conclusion, the bacteriocin produced by P. pentosaceus SB83 has been proved to be resistant to several conditions, including conditions in the vaginal tract (pH and components of vaginal fluid).por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationBORGES, Sandra … [et al.] - Characterization of a bacteriocin of pediococcus pentosaceus SB83 and its potential for vaginal application. Anti-Infective Agents. ISSN 2211-3533. Vol. 12, n.º 1 (2014), p. 68-74por
dc.identifier.doi10.2174/22113525113119990113
dc.identifier.issn1871-5214
dc.identifier.urihttp://hdl.handle.net/10400.14/17601
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationStrategic Project - LA 16 - 2011-2012
dc.subjectAntimicrobial activitypor
dc.subjectBacteriocinpor
dc.subjectNeonatal listeriosispor
dc.subjectPregnancypor
dc.subjectVaginal applicationpor
dc.subjectVaginal fluidpor
dc.titleCharacterization of a bacteriocin of pediococcus pentosaceus SB83 and its potential for vaginal applicationpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F45496%2F2008/PT
oaire.citation.endPage74
oaire.citation.issue1
oaire.citation.startPage68
oaire.citation.titleAnti-Infective Agents
oaire.citation.volume12
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameBorges
person.familyNameBarbosa
person.familyNameSilva
person.familyNameTeixeira
person.givenNameSandra
person.givenNameJoana
person.givenNameJoana
person.givenNamePaula
person.identifier.ciencia-id591F-66D5-8F90
person.identifier.ciencia-id9516-589F-8B0D
person.identifier.ciencia-idB415-743D-D9F9
person.identifier.orcid0000-0003-4665-1098
person.identifier.orcid0000-0001-5879-9458
person.identifier.orcid0000-0003-3977-7418
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id15826021600
person.identifier.scopus-author-id13608752600
person.identifier.scopus-author-id7403023130
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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