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Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions

2022-12-07Journal article Open access
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dc.contributor.authorMachado, Daniela
dc.contributor.authorDomingos, Melany
dc.contributor.authorBarbosa, Joana Cristina
dc.contributor.authorAlmeida, Diana
dc.contributor.authorAndrade, José Carlos
dc.contributor.authorFreitas, Ana Cristina
dc.contributor.authorGomes, Ana Maria
dc.date.accessioned2022-12-21T18:03:43Z
dc.date.available2022-12-21T18:03:43Z
dc.date.issued2022-12-07
dc.description.abstractFaecalibacterium duncaniae is an intestinal commensal bacterium proposed as a next-generation probiotic due to its promising outcomes in the treatment and prevention of several human diseases, which demonstrate its multiple contributions to the host’s health. However, its strict anaerobic nature has created several hurdles in the development of functional foods, nutraceuticals, and biotherapeutic products. Herein, we explored freeze-dried formulations containing prebiotics, cryoprotectants, and antioxidant agents as a technological strategy to enhance the viability of F. duncaniae DSM 17677 upon aerobic storage and gastrointestinal tract conditions. Our results indicate that freeze-dried F. duncaniae in a matrix containing inulin, sucrose, cysteine, and riboflavin survived at levels higher than 106 CFU/g and around 105 CFU/g after 1 and 4 days of aerobic storage at room temperature, respectively. Thus, the freeze-dried formulation with inulin, sucrose, cysteine, and riboflavin presents as a protective strategy to improve F. duncaniae viability under aerobic environments. Nevertheless, incorporation of a suitable coating aimed at protecting F. duncaniae against the detrimental gastrointestinal passage effects is urgently required, given its high susceptibility to extreme acidic pH values and bile.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/pharmaceutics14122735pt_PT
dc.identifier.eid85144846100
dc.identifier.issn1999-4923
dc.identifier.pmcPMC9785611
dc.identifier.pmid36559229
dc.identifier.urihttp://hdl.handle.net/10400.14/39633
dc.identifier.wos000904187600001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAcidic pH susceptibilitypt_PT
dc.subjectAerobic storagept_PT
dc.subjectAntioxidantspt_PT
dc.subjectBile susceptibilitypt_PT
dc.subjectCryoprotectantspt_PT
dc.subjectFaecalibacterium duncaniaept_PT
dc.subjectFreeze-dryingpt_PT
dc.subjectGastrointestinal conditionspt_PT
dc.subjectNext-generation probioticspt_PT
dc.subjectViabilitypt_PT
dc.titleExploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00520%2F2017%2FCP1404%2FCT0001/PT
oaire.citation.issue12pt_PT
oaire.citation.titlePharmaceuticspt_PT
oaire.citation.volume14pt_PT
oaire.fundingStreamCEEC IND 2017
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication077fbbcc-1b7e-49e5-a895-ad5c9f4fc572
relation.isProjectOfPublication.latestForDiscovery077fbbcc-1b7e-49e5-a895-ad5c9f4fc572

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