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Phytosterols and novel triterpenes recovered from industrial fermentation coproducts exert in vitro anti‐inflammatory activity in macrophages

2021-06Journal article Open access
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dc.contributor.authorTeixeira, Francisca S.
dc.contributor.authorVidigal, Susana S. M. P.
dc.contributor.authorPimentel, Lígia L.
dc.contributor.authorCosta, Paula T.
dc.contributor.authorTavares‐Valente, Diana
dc.contributor.authorAzevedo‐Silva, João
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorFernandes, João C.
dc.contributor.authorRodríguez‐Alcalá, Luís M.
dc.date.accessioned2021-07-06T11:42:50Z
dc.date.available2021-07-06T11:42:50Z
dc.date.issued2021-06
dc.description.abstractThe unstoppable growth of human population that occurs in parallel with all manufacturing activities leads to a relentless increase in the demand for resources, cultivation land, and energy. In response, currently, there is significant interest in developing strategies to optimize any available resources and their biowaste. While solutions initially focused on recovering biomolecules with applications in food, energy, or materials, the feasibility of synthetic biology in this field has been demonstrated in recent years. For instance, it is possible to genetically modify Saccharomyces cerevisiae to produce terpenes for commercial applications (i.e., against malaria or as biodiesel). But the production process, similar to any industrial activity, generates biowastes containing promising biomolecules (from fermentation) that if recovered may have applications in different areas. To test this hypothesis, in the present study, the lipid composition of by‐products from the industrial production of β‐farnesene by genetically modified Saccharomyces cerevisiae are studied to identify potentially bioactive compounds, their recovery, and finally, their stability and in vitro bioactivity. The assayed biowaste showed the presence of triterpenes, phytosterols, and 1‐ octacosanol which were recovered through molecular distillation into a single fraction. During the assayed stability test, compositional modifications were observed, mainly for the phytosterols and 1‐octacosanol, probably due to oxidative reactions. However, such changes did not affect the in vitro bioactivity in macrophages, where it was found that the obtained fraction decreased the production of TNF‐α and IL‐6 in lipopolysaccharide (LPS)‐induced inflammation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ph14060583pt_PT
dc.identifier.eid85108866914
dc.identifier.issn1424-8247
dc.identifier.pmcPMC8235040
dc.identifier.pmid34207156
dc.identifier.urihttp://hdl.handle.net/10400.14/34069
dc.identifier.wos000665880900001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subject1‐octacosanolpt_PT
dc.subjectBiowastept_PT
dc.subjectIL‐6pt_PT
dc.subjectInflammationpt_PT
dc.subjectMacrophagespt_PT
dc.subjectMolecular distillationpt_PT
dc.subjectPhytosterolspt_PT
dc.subjectStability testpt_PT
dc.subjectTriterpenespt_PT
dc.titlePhytosterols and novel triterpenes recovered from industrial fermentation coproducts exert in vitro anti‐inflammatory activity in macrophagespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6pt_PT
oaire.citation.titlePharmaceuticalspt_PT
oaire.citation.volume14pt_PT
person.familyNameVidigal
person.familyNamePimentel
person.familyNamePintado
person.familyNameFernandes
person.givenNameSusana
person.givenNameLígia
person.givenNameMaria Manuela
person.givenNameJoão
person.identifier534450
person.identifier456608
person.identifier.ciencia-id2919-B77C-11C0
person.identifier.ciencia-id6618-9DD9-3C65
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-id881D-7FFD-DD7D
person.identifier.orcid0000-0002-0213-063X
person.identifier.orcid0000-0002-6656-017X
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-1556-1698
person.identifier.ridM-4661-2013
person.identifier.ridK-3254-2014
person.identifier.ridF-5696-2013
person.identifier.ridK-3126-2013
person.identifier.scopus-author-id11739488200
person.identifier.scopus-author-id22035944100
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id35274726800
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationd6520be9-873a-41f9-9aa1-96a5b65e8070
relation.isAuthorOfPublicationba387c7d-27c9-4016-895c-b35597e91ebc
relation.isAuthorOfPublication4f081a71-52de-47ab-80f1-45a546c3ebab
relation.isAuthorOfPublication.latestForDiscovery59453cc2-ee50-4a1d-bbfc-924ca6417004

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