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Monitoring alcoholic fermentation: an untargeted approach

dc.contributor.authorFerreira, António César Silva
dc.contributor.authorMonforte, Ana Rita
dc.contributor.authorTeixeira, Carla Silva
dc.contributor.authorMartins, Rosa
dc.contributor.authorFairbaim, Samantha
dc.contributor.authorBauer, Florian F.
dc.date.accessioned2015-04-28T16:46:23Z
dc.date.available2015-04-28T16:46:23Z
dc.date.issued2014
dc.description.abstractThis work describes the utility and efficiency of a metabolic profiling pipeline that relies on an unsupervised and untargeted approach applied to a HS-SPME/GC-MS data. This noninvasive and high throughput methodology enables "real time" monitoring of the metabolic changes inherent to the biochemical dynamics of a perturbed complex biological system and the extraction of molecular candidates that are latter validated on its biochemical context. To evaluate the efficiency of the pipeline five different fermentations, carried on a synthetic media and whose perturbation was the nitrogen source, were performed in 5 and 500 mL. The smaller volume fermentations were monitored online by HS-SPME/GC-MS, allowing to obtain metabolic profiles and molecular candidates time expression. Nontarget analysis was applied using MS data in two ways: (i) one dimension (1D), where the total ion chromatogram per sample was used, (ii) two dimensions (2D), where the integrity time vs m/z per sample was used. Results indicate that the 2D procedure captured the relevant information more efficiently than the 1D. It was also seen that although there were differences in the fermentation performance in different scales, the metabolic pathways responsible for production of metabolites that impact the quality of the volatile fraction was unaffected, so the proposed pipeline is suitable for the study of different fermentation systems that can undergo subsequent sensory validation on a larger scale.en
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationSILVA FERREIRA, António César ...[et al.] - Monitoring Alcoholic Fermentation: An Untargeted Approach. Journal of Agricultural and Food Chemistry. ISSN 0021-8561. Vol. 62 (2014), 6784−6793por
dc.identifier.doi10.1021/jf502082z
dc.identifier.eid84904430262
dc.identifier.pmid24976138
dc.identifier.urihttp://hdl.handle.net/10400.14/17367
dc.identifier.wos000339226700034
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Chemical Society
dc.subjectMetabolomicpor
dc.subjectFermentationpor
dc.subjectTargetpor
dc.subjectUntargetedpor
dc.subjectHS-SPME/GC-MS, PCApor
dc.subjectPCApor
dc.subjectOPLS-DApor
dc.titleMonitoring alcoholic fermentation: an untargeted approachpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-ALI%2F121062%2F2010/PT
oaire.citation.endPage6793
oaire.citation.issue28
oaire.citation.startPage6784
oaire.citation.titleJournal of Agricultural and Food Chemistry
oaire.citation.volume62
oaire.fundingStream3599-PPCDT
person.familyNameSilva Ferreira
person.familyNameMonforte
person.familyNameSilva Teixeira
person.familyNameMartins
person.familyNameBauer
person.givenNameAntonio Cesar
person.givenNameAna Rita
person.givenNameCarla Sílvia
person.givenNameRosa
person.givenNameFlorian Franz
person.identifier265800
person.identifier.ciencia-id931A-48DA-23A8
person.identifier.ciencia-id471B-600E-ABC5
person.identifier.ciencia-id3211-BAEF-3FB0
person.identifier.ciencia-id051A-5A02-E227
person.identifier.orcid0000-0002-1188-1021
person.identifier.orcid0000-0003-2627-2656
person.identifier.orcid0000-0002-8422-9392
person.identifier.orcid0000-0003-1842-4840
person.identifier.orcid0000-0001-5764-4542
person.identifier.ridK-7773-2014
person.identifier.scopus-author-id55622494400
person.identifier.scopus-author-id57160292700
person.identifier.scopus-author-id56275265400
person.identifier.scopus-author-id7202999387
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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