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Enzymatic hydrolysates from Fucus vesiculosus: optimal process, chemical profile and bioactivity

2026-07-18Artigo de investigação Acesso aberto
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dc.contributor.authorNova, Paulo
dc.contributor.authorCoelho, Marta
dc.contributor.authorCunha, Sara A.
dc.contributor.authorMachado, Manuela
dc.contributor.authorCosta-Pinto, Ana R.
dc.contributor.authorGomes, Ana Maria
dc.date.accessioned2026-08-06T15:24:19Z
dc.date.available2026-08-06T15:24:19Z
dc.date.issued2026-07-18
dc.description.abstractFucus vesiculosus (FV) is a brown macroalga rich in bioactive compounds with significant industrial potential. This study aimed to produce enzyme-assisted water-soluble hydrolysates from FV with optimized antioxidant activity using Box–Behnken experimental designs. Two extraction methods were evaluated: cellulase alone (FVc) and a combination of cellulase and alcalase (FVca). The optimization focused on enzyme concentration, temperature, and incubation time, measuring extraction yield, total phenolic content as determined by the Folin–Ciocalteu assay (non-specific reducing capacity index, FC-derived TPC), total antioxidant capacity (TAC), and oxygen radical absorbance capacity (ORAC). Results demonstrated that the combined dual-enzyme (FVca) treatment was highly efficient, simultaneously maximizing the extraction yield (39.41%) and the overall reducing capacity (TAC of 142.80 µmol TE/g, ORAC of 477.64 µmol TE/g, and a FC-derived TPC of 252.57 mg GAE/g). Due to its higher potential, FVca was further characterized, revealing a rich profile of essential amino acids, low-molecular-weight peptides, and a diverse phenolic profile, dominated by phloroglucinol (6.23 mg/g). In addition, the FVca hydrolysate demonstrated severe abiotic interference with the redox viability assay at 10 mg/mL in Caco-2 human colorectal adenocarcinoma cell cultures. These findings highlight that combining cellulase and alcalase effectively solubilizes key bioactive compounds, yielding a hydrolysate highly promising for industrial and biotechnological applications.eng
dc.identifier.doi10.3390/md24070251
dc.identifier.eid105045788213
dc.identifier.other91749ed2-bcb6-4471-8148-7f1373be57a8
dc.identifier.pmcPMC13412148
dc.identifier.pmid42505991
dc.identifier.urihttp://hdl.handle.net/10400.14/59027
dc.identifier.wos001832533800001
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCarbohydraseseng
dc.subjectEnzyme-assisted extractioneng
dc.subjectExtractioneng
dc.subjectMacroalgaeeng
dc.subjectProteaseseng
dc.subjectValue added compoundseng
dc.titleEnzymatic hydrolysates from Fucus vesiculosus: optimal process, chemical profile and bioactivity
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.issue7
oaire.citation.volume24
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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