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Characterization of TGF-β expression and signaling profile in the adipose tissue of rats fed with high-fat and energy-restricted diets

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dc.contributor.authorSousa-Pinto, Bernardo
dc.contributor.authorGonçalves, Laura
dc.contributor.authorRodrigues, Adriana R.
dc.contributor.authorTomada, Inês
dc.contributor.authorAlmeida, Henrique
dc.contributor.authorNeves, Delminda
dc.contributor.authorGouveia, Alexandra M.
dc.date.accessioned2017-10-11T14:54:09Z
dc.date.available2017-10-11T14:54:09Z
dc.date.issued2016
dc.description.abstractTransforming growth factor beta (TGF-β) plays an important role in the pathogenesis of obesity, influencing the release of inflammation mediators and promoting remodeling and collagen deposition in the adipose tissue (AT). In this context, this work aims to elucidate whether TGF-β and Smad-dependent or Smad-independent signaling pathways contribute to regional differentiation of AT in high-fat diet (HFD) and energy-restricted (ER) rat models. For this, TGF-β, TGF-β receptors I and II, PAI-1 and GLUT4 mRNA levels were quantified by real-time PCR, and western blotting assays allowed the semiquantification of TGF-β and proteins from Smad3, ERK1/2 and Akt signaling pathways in subcutaneous and visceral (epididymal, retroperitoneal and mesenteric) fat depots from control, HFD and ER-treated rats. HFD was associated to increased levels of TGF-β and PAI-1 mRNA in epididymal and retroperitoneal visceral fat depots, while ER diet induced a reduction of TGF-β mRNA levels in mesenteric, but surprisingly an increase in retroperitoneal fat. Regarding the different signaling pathways, contrarily to what was found for Smad3, activation of ERK1/2 and Akt in response to HFD was detected in all the visceral but not in subcutaneous fat depots. ER-treated rats presented a more heterogeneous signaling response, as well as decreased TGF-β receptors expression, in the different visceral fat depots. In conclusion, subcutaneous and visceral AT respond differently to distinct diet patterns regarding TGF-β expression and activated signaling pathways. Furthermore, the present study points that visceral AT should not be understood as a homogeneous entity since that response also varied in the different fat depots.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSOUSA-PINTO, Bernardo; GONCALVES, Laura; RODRIGUES, Adriana R.; TOMADA, Ines; ALMEIDA, Henrique; NEVES, Delminda; GOUVEIA, Alexandra M. - Characterization of TGF-beta expression and signaling profile in the adipose tissue of rats fed with high-fat and energy-restricted diets. Journal Of Nutritional Biochemistry. ISSN 1873-4847. Vol. 38 (2016), p. 107-115pt_PT
dc.identifier.doi10.1016/j.jnutbio.2016.07.017pt_PT
dc.identifier.eid84991503722
dc.identifier.pmid27736730
dc.identifier.urihttp://hdl.handle.net/10400.14/22964
dc.identifier.wos000388431000013
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.subjectTransforming growth factor betapt_PT
dc.subjectAdipose tissuept_PT
dc.subjectEnergy restrictionpt_PT
dc.subjectHigh-fat dietpt_PT
dc.subjectObesitypt_PT
dc.subjectSignaling pathwayspt_PT
dc.titleCharacterization of TGF-β expression and signaling profile in the adipose tissue of rats fed with high-fat and energy-restricted dietspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F92868%2F2013/PT
oaire.citation.endPage115
oaire.citation.startPage107
oaire.citation.titleJournal of Nutritional Biochemistrypt_PT
oaire.citation.volume38
oaire.fundingStreamSFRH
person.familyNameSousa-Pinto
person.familyNameRodrigues
person.familyNameTomada
person.familyNameAlmeida
person.familyNameNeves
person.familyNameGouveia
person.givenNameBernardo
person.givenNameAdriana
person.givenNameInês
person.givenNameHenrique
person.givenNameDelminda
person.givenNameAlexandra
person.identifier2689332
person.identifier.ciencia-id1510-E111-1980
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person.identifier.ciencia-id7719-7A32-37A7
person.identifier.ciencia-id0D19-14A5-3B1B
person.identifier.ciencia-id8C17-D9AB-804A
person.identifier.ciencia-id2C15-98D1-4FA9
person.identifier.orcid0000-0002-1277-3401
person.identifier.orcid0000-0002-9613-1552
person.identifier.orcid0000-0003-4660-0645
person.identifier.orcid0000-0002-3036-3211
person.identifier.orcid0000-0002-3301-8376
person.identifier.orcid0000-0001-8784-4246
person.identifier.ridO-2846-2014
person.identifier.ridP-3525-2016
person.identifier.scopus-author-id55982726300
person.identifier.scopus-author-id25223739100
person.identifier.scopus-author-id25223994400
person.identifier.scopus-author-id23017711100
person.identifier.scopus-author-id8148778800
person.identifier.scopus-author-id7005609395
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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