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Polymeric nanoparticles as oral delivery systems for a grape pomace extract towards the improvement of biological activities

2021Journal article Restricted access
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dc.contributor.authorCosta, Joana R.
dc.contributor.authorXavier, Miguel
dc.contributor.authorAmado, Isabel R.
dc.contributor.authorGonçalves, Catarina
dc.contributor.authorCastro, Pedro M.
dc.contributor.authorTonon, Renata V.
dc.contributor.authorCabral, Lourdes M.C.
dc.contributor.authorPastrana, Lorenzo
dc.contributor.authorPintado, Manuela E.
dc.date.accessioned2020-10-13T13:43:58Z
dc.date.available2020-10-13T13:43:58Z
dc.date.issued2021
dc.description.abstractGrape pomace (GP) is a major by-product from the wine industry, known for its bioactive compounds and their impact upon gastrointestinal (GI) health. However, bioaccessibility is often poor due to their degradation during digestion. This work aimed to encapsulate bioactive GP extract (GPE) into chitosan (CS) and alginate (Alg) nanoparticles (NPs) to mitigate degradation in the GI tract. Alg and CS NPs were optimized using a rotatable central composite design and NPs were characterized for their size, polydispersity, zeta potential and total phenolics (TP) association efficiency. The best formulations showed sizes ranging 523–853 nm, polydispersity indexes of 0.11–0.36, zeta potential of −15.0–14.9 mV and TP association efficiencies of 68 and 65%. FTIR confirmed that there was no formation of new chemical groups after association of the polymers with GPE. Both formulations improved the bioaccessibility of different phenolics following in vitro GI digestion, leading to in-creased antioxidant and antimicrobial activities. Moreover, the permeability of bioactive compounds through a Caco-2/HT29-MTX co-culture was reduced, suggesting a higher residence time in the intestine. Cy5.5 was used for tracking the CS NPs, which did not affect the metabolic activity of Caco-2 and HT29-MTX cells. Confocal microscopy images confirmed the adsorption of NPs to the cellular layer and suggested a reduction of the tight junction protein occludin when cells were incubated with Cy5.5-CS in solution. This study suggests that encapsulation of GPE can offer protection against along the GI tract and improve its biological activity with significant impact for oral delivery applications, including functional foods.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta, J. R., Xavier, M., Amado, I. R., Gonçalves, C., Castro, P. M., Tonon, R. V., ... Pintado, M. E. (2020). Polymeric nanoparticles as oral delivery systems for a grape pomace extract towards the improvement of biological activities. Materials Science and Engineering: C, 119, art. n.º 111551pt_PT
dc.identifier.doi10.1016/j.msec.2020.111551pt_PT
dc.identifier.eid85092049365
dc.identifier.issn0928-4931
dc.identifier.pmid33321615
dc.identifier.urihttp://hdl.handle.net/10400.14/31095
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-0145-FEDER-01640pt_PT
dc.relationCentre for Biotechnology and Fine Chemistry
dc.subjectIndustrial by-productpt_PT
dc.subjectEncapsulationpt_PT
dc.subjectBioaccessibilitypt_PT
dc.subjectAntioxidantpt_PT
dc.subjectAntimicrobialpt_PT
dc.subjectCellular uptakept_PT
dc.titlePolymeric nanoparticles as oral delivery systems for a grape pomace extract towards the improvement of biological activitiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.citation.titleMaterials Science and Engineering: Cpt_PT
oaire.citation.volume119pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRibeiro Costa
person.familyNameMiranda de Castro
person.familyNamePintado
person.givenNameJoana
person.givenNamePedro João
person.givenNameMaria Manuela
person.identifier456608
person.identifier.ciencia-id5112-6B99-DC1F
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-0559-0540
person.identifier.orcid0000-0001-7164-8005
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id57201808340
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication99909754-7408-4e67-8f02-9cdb3c5a88d4
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