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Chitosan nanoparticles for daptomycin delivery in ocular treatment of bacterial endophthalmitis

2015-10-03Journal article Restricted access
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dc.contributor.authorSilva, Nádia C.
dc.contributor.authorSilva, Sara
dc.contributor.authorSarmento, Bruno
dc.contributor.authorPintado, Manuela
dc.date.accessioned2021-07-06T19:02:25Z
dc.date.available2021-07-06T19:02:25Z
dc.date.issued2015-10-03
dc.description.abstractContext: Chitosan nanoparticles were prepared to encapsulate daptomycin and proposed as a delivery system of this antibiotic to the eye for the treatment of bacterial endophthalmitis. Objective: The aim of this study was to develop daptomycin-loaded nanoparticles to apply directly to the eye, as a possible non-invasive and less painful alternative for the treatment of endophthalmitis, increasing the effectiveness of treatment and reducing toxicity associated with systemic administration.Materials and methods: Nanoparticles were obtained by ionotropic gelation between chitosan and sodium tripolyphosphate (TPP). Physicochemical and morphological characteristics of nanoparticles were evaluated, as well as determination of antimicrobial efficiency of encapsulated daptomycin and stability of the nanoparticles in the presence of lysozyme and mucin.Results: Loaded nanoparticles presented mean particle sizes around 200 nm, low polydispersity index, and positive zeta potential. Morphological examination by scanning electron microscopy (SEM) confirmed their small size and round-shaped structure. Encapsulation efficiency ranged from 80 to 97%. Total in vitro release of daptomycin was obtained within 4 h. Determination of minimum inhibitory concentrations (MICs) showed that bacteria were still susceptible to daptomycin encapsulated into the nanoparticles. Incubation with lysozyme did not significantly affect the integrity of the nanoparticles, although mucin positively affected their mucoadhesive properties. Discussion and conclusion: The obtained nanoparticles have suitable characteristics for ocular applications, arising as a promising solution for the topical administration of daptomycin to the eye.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3109/10717544.2013.858195pt_PT
dc.identifier.eid84942930709
dc.identifier.issn1071-7544
dc.identifier.pmid24266551
dc.identifier.urihttp://hdl.handle.net/10400.14/34099
dc.identifier.wos000369752800001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectAntibacterial activitypt_PT
dc.subjectChitosanpt_PT
dc.subjectDaptomycinpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectOcular infectionpt_PT
dc.titleChitosan nanoparticles for daptomycin delivery in ocular treatment of bacterial endophthalmitispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage893pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage885pt_PT
oaire.citation.titleDrug Deliverypt_PT
oaire.citation.volume22pt_PT
person.familyNameSilva
person.familyNameSilva
person.familyNameSarmento
person.familyNamePintado
person.givenNameNádia
person.givenNameSara
person.givenNameBruno
person.givenNameMaria Manuela
person.identifier1888852
person.identifier152609
person.identifier456608
person.identifier.ciencia-idD215-90DC-618A
person.identifier.ciencia-id8E18-0AFF-BF6D
person.identifier.ciencia-idE816-B6B0-06A5
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-2090-4923
person.identifier.orcid0000-0001-8268-2137
person.identifier.orcid0000-0001-5763-7553
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridV-9872-2017
person.identifier.ridJ-6265-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id41662079600
person.identifier.scopus-author-id55158300800
person.identifier.scopus-author-id57194334236
person.identifier.scopus-author-id7004483898
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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