Publication
Chemical and antioxidant properties of solvent and enzyme-assisted extracts of Fucus vesiculosus and Porphyra dioica
| dc.contributor.author | Nova, Paulo | |
| dc.contributor.author | Cunha, Sara A. | |
| dc.contributor.author | Costa-Pinto, Ana R. | |
| dc.contributor.author | Gomes, Ana Maria | |
| dc.date.accessioned | 2024-08-08T10:11:37Z | |
| dc.date.available | 2024-08-08T10:11:37Z | |
| dc.date.issued | 2024-07-18 | |
| dc.description.abstract | Extraction strategies impact the efficiency and nature of extracted compounds. This work assessed the chemical composition and antioxidant capacity of ethanolic, hydroethanolic, and aqueous versus enzyme-assisted extracts (isolated or with the sequential use of alcalase®, cellulase®, and viscozyme®) of the macroalgae Fucus vesiculosus (brown, Phaeophyceae) and Porphyra dioica (red, Rhodophyta. For both macroalgae, enzyme-assisted extraction (EAE) was the most efficient process compared to solvent-assisted extraction (SAE), independent of solvent. Fucus vesiculosus extraction yields were higher for EAE than for SAE (27.4% to 32.2% and 8.2% to 30.0%, respectively). Total phenolics content (TPC) was at least 10-fold higher in EAE extracts (229.2 to 311.3 GAE/gextract) than in SAE (4.34 to 19.6 GAE/gextract) counterparts and correlated well with antioxidant capacity (ABTS and ORAC methods), with EAE achieving values up to 8- and 2.6-fold higher than those achieved by SAE, respectively. Porphyra dioica followed F. vesiculosus’s trend for extraction yields (37.5% to 51.6% for EAE and 5.7% to 35.1% for SAE), TPC, although of a lower magnitude, (0.77 to 8.95 GAE/gextract for SE and 9.37 to 14.73 GAE/gextract for EAE), and antioxidant capacity. Aqueous extracts registered the highest DPPH values for both macroalgae, with 2.3 µmol TE/gextract and 13.3 µmol TE/gextract for F. vesiculosus and P. dioica, respectively. EAE was a more efficient process in the extraction of soluble protein and reducing sugars in comparison to SAE. Furthermore, an improved effect of enzyme-assisted combinations was observed for almost all analyzed parameters. This study shows the promising application of enzyme-assisted extraction for the extraction of valuable compounds from F. vesiculosus and P.dioica, making them excellent functional ingredients for a wide range of health and food industrial applications. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.3390/md22070319 | pt_PT |
| dc.identifier.eid | 85199608919 | |
| dc.identifier.issn | 1660-3397 | |
| dc.identifier.pmc | PMC11278442 | |
| dc.identifier.pmid | 39057428 | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/46111 | |
| dc.identifier.wos | 001278538400001 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Carbohydrases | pt_PT |
| dc.subject | Enzyme-assisted extraction | pt_PT |
| dc.subject | Extraction | pt_PT |
| dc.subject | Macroalgae | pt_PT |
| dc.subject | Proteases | pt_PT |
| dc.subject | Value-added compounds | pt_PT |
| dc.title | Chemical and antioxidant properties of solvent and enzyme-assisted extracts of Fucus vesiculosus and Porphyra dioica | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 7 | pt_PT |
| oaire.citation.title | Marine Drugs | pt_PT |
| oaire.citation.volume | 22 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |