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Characterization of clinical and food Listeria monocytogenes isolates with different antibiotic resistance patterns through simulated gastrointestinal tract conditions and environmental stresses

2016Journal article Restricted access
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dc.contributor.authorCunha, S.
dc.contributor.authorKomora, N.
dc.contributor.authorMagalhães, R.
dc.contributor.authorAlmeida, Gonçalo
dc.contributor.authorFerreira, V.
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2016-04-21T13:14:16Z
dc.date.available2016-04-21T13:14:16Z
dc.date.issued2016
dc.description.abstractThirty-three Listeria monocytogenes isolates previously collected from two sources, food (n = 18) and human patients suffering from listeriosis (n = 15), with variable antibiotic susceptibility profiles (sensitive/resistant) to erythromycin, ciprofloxacin and nitrofurantoin, were studied for their ability to survive (i) the environmental stress provided by sequential conditions that simulate the digestive tract, and (ii) extreme pH values (1.5–12). The results showed a response that was only strain dependent. There were no variability in survival results based on type of stress (low or high pH), source (food or clinical), or sensitivity/resistance to antibiotics (p > 0.01). Some strains of L. monocytogenes are able to survive extreme acid and alkaline conditions, and conditions that mimic the sequential stressors found in the gastro-intestinal tract. The resistance to the antibiotics tested in this study by some L. monocytogenes strains did not confer any cross-protection to acid or alkaline stressors.pt_PT
dc.description.sponsorshiphis work was supported by funding from the National Funds from the Fundação para a Ciência e a Tecnologia (FCT) through project Pest-OE/EQB/LA0016/2013 and “KLEAR - Knowledge and tools for safety, quality and competitiveness in the food chain.” n º NORTE-07-0124-FEDER-000052, co-funded by Fundo Europeu de Desenvolvimento Regional (FEDER), under Programa ON2, Eixo Prior- itário 01-01 – Competitividade, Inovação e Conhecimento, Tipologia 01-01-01-24 – Apoio a Entidades do Sistema Científico e Tecnológico. Financial support for authors Rui Magalhães and Vânia Ferreira was provided by FCT through doctoral fellowship SFRH/BD/71704/2010 and postdoctoral fellowship SFRH/BPD/72617/2010, respectively. Editing of this paper by Dr. P. A. Gibbs is gratefully acknowledgedpt_PT
dc.identifier.citationCUNHA, S.; KOMORA, N.; MAGALHÃES, R.; ALMEIDA, Gonçalo; FERREIRA, V.; TEIXEIRA, Paula - Characterization of clinical and food Listeria monocytogenes isolates with different antibiotic resistance patterns through simulated gastrointestinal tract conditions and environmental stresses. Microbial Risk Analysis. ISSN 2352-3522. Vol. 1 (2016), p. 40-60 http://dx.doi.org/10.1016/j.mran.2015.08.001pt_PT
dc.identifier.eid84953295776
dc.identifier.urihttp://hdl.handle.net/10400.14/19889
dc.identifier.wos000503367000006
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationNORTE-07-0124-FEDER-0000pt_PT
dc.relationCHARACTERISTICS OF SPECIFIC STRAINS OF LISTERIA MONOCYTOGENES POTENTIATING PERSISTENCE IN FOOD AND FOOD PROCESSING ENVIRONMENTS AND RELEVANCE TO FOOD SAFETY
dc.subjectListeria monocytogenespt_PT
dc.subjectAcid stresspt_PT
dc.subjectAlkali stresspt_PT
dc.subjectGastro-intestinaltractpt_PT
dc.subjectAntibiotic resistancept_PT
dc.titleCharacterization of clinical and food Listeria monocytogenes isolates with different antibiotic resistance patterns through simulated gastrointestinal tract conditions and environmental stressespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCHARACTERISTICS OF SPECIFIC STRAINS OF LISTERIA MONOCYTOGENES POTENTIATING PERSISTENCE IN FOOD AND FOOD PROCESSING ENVIRONMENTS AND RELEVANCE TO FOOD SAFETY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F71704%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F72617%2F2010/PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameKomora
person.familyNameMagalhães
person.familyNameAlmeida
person.familyNameBorges Ferreira
person.familyNameTeixeira
person.givenNameNorton
person.givenNameRui
person.givenNameGonçalo
person.givenNameVânia
person.givenNamePaula
person.identifierK-1359-2014
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person.identifier.orcid0000-0002-1118-3629
person.identifier.orcid0000-0003-3172-440X
person.identifier.orcid0000-0003-1513-0951
person.identifier.orcid0000-0001-7093-9193
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridK-1359-2014
person.identifier.ridK-4389-2014
person.identifier.ridK-1343-2014
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id57039111500
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person.identifier.scopus-author-id7003759404
person.identifier.scopus-author-id8317338900
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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