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Effect of different conditions of growth and storage on the cell counts of two lactic acid bacteria after spray drying in orange juice

dc.contributor.authorBarbosa, Joana
dc.contributor.authorBorges, Sandra
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2017-10-17T08:59:55Z
dc.date.available2017-10-17T08:59:55Z
dc.date.issued2016
dc.description.abstractConsumers increasingly require innovative food products with health benefits. Thus, a dried orange juice incorporating probiotics could be a novel challenge. In this context, we investigated whether different sugars added to the culture media used for growth of two lactic acid bacteria contributed to their protection during spray drying in orange juice and subsequent storage under different conditions of temperature, light exposure and water activity. Cell viability during passage through simulated gastro-intestinal conditions was also investigated. Cells grown in culture medium containing fructose resulted in the worst survival rates during storage. High survival was observed for cells grown in the presence of lactose, followed by glucose. The survival of dried bacteria was enhanced at 4 C, water activity of 0.03 and absence of daylight. For cells grown in standard culture medium and after 12 months of storage at 4 C in orange juice powder (about 109 cfu/mL), there was a reduction of approximately 2 log-units for both lactic acid bacteria after gastro-intestinal tract passage simulation. Using the conditions of growth and storage investigated, it is possible to improve the survival rate of lactic acid bacteria and produce an orange juice powder with probiotic characteristics with shelf life of at least 12 months.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBARBOSA, Joana; BORGES, Sandra; TEIXEIRA, Paula - Effect of Different Conditions of Growth and Storage on the Cell Counts of Two Lactic Acid Bacteria after Spray Drying in Orange Juice. Beverages. ISSN 2306-5710. Vol. 2, n.º 2 (2016), 12 p.pt_PT
dc.identifier.doi10.3390/beverages2020008pt_PT
dc.identifier.eid85045548377
dc.identifier.urihttp://hdl.handle.net/10400.14/23129
dc.identifier.wos000455131800001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCentre for Biotechnology and Fine Chemistry
dc.relationLISTERIA MONOCYTOGENES IN FOODS: CONTRIBUTING DATA FOR RISK ASSESSMENT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGastro-intestinal tractpt_PT
dc.subjectLactobacillus plantarum 299vpt_PT
dc.subjectPediococcus acidilactici HA-6111-2pt_PT
dc.subjectProbiotic juicept_PT
dc.subjectSugarspt_PT
dc.titleEffect of different conditions of growth and storage on the cell counts of two lactic acid bacteria after spray drying in orange juicept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardTitleLISTERIA MONOCYTOGENES IN FOODS: CONTRIBUTING DATA FOR RISK ASSESSMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F48894%2F2008/PT
oaire.citation.issue2
oaire.citation.titleBeveragespt_PT
oaire.citation.volume2
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBarbosa
person.familyNameBorges
person.familyNameTeixeira
person.givenNameJoana
person.givenNameSandra
person.givenNamePaula
person.identifier.ciencia-id9516-589F-8B0D
person.identifier.ciencia-id591F-66D5-8F90
person.identifier.orcid0000-0001-5879-9458
person.identifier.orcid0000-0003-4665-1098
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id13608752600
person.identifier.scopus-author-id15826021600
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication.latestForDiscoveryce89919c-98a7-473c-995a-1d89e70644b6
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