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Evolution of gentamicin and arsenite resistance acquisition in Ralstonia pickettii water isolates

2021Journal article Restricted access
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dc.contributor.authorFerro, Pompeyo
dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2020-12-11T18:10:03Z
dc.date.available2020-12-11T18:10:03Z
dc.date.issued2021
dc.description.abstractRalstonia pickettii are ubiquitous in water environments. Members of this species are frequently, but not always, resistant to both gentamicin and arsenite. Gentamicin and arsenite co-resistance and the putative molecular mechanisms were investigated. A group of 37 R. pickettii strains isolated from drinking water and hospital wastewater were characterized for gentamicin and arsenite resistance phenotypes, the number and size of plasmids, and screened for genetic elements associated with arsenite tolerance, Integrative and Conjugative Elements (ICEs), among other. The genomes of three representative strains were compared. Most gentamicin resistant (GR) isolates (32/33) were resistant to arsenite, and harbored ICE- and ars operon-related genes. These genetic elements were not detected in any of the five arsenite susceptible strains, regardless of the GR (n = 1) or gentamicin susceptibility (GS) (n = 4) phenotype. The comparison of the genomes of two GR (one resistant and one susceptible to arsenite) and one GS strains suggested that these phenotypes correspond to three phylogroups, distinguished by presence of some genes only in GR isolates, in addition to point mutations in functional genes. The presence of ICEs and ars operon-related genes suggest that arsenite resistance might have been acquired by GR lineages.pt_PT
dc.description.versioninfo:eu-repo/semantics/aceptedVersionpt_PT
dc.identifier.citationFerro, P., Vaz-Moreira, I., Manaia, C.M. (2021). Evolution of gentamicin and arsenite resistance acquisition in Ralstonia pickettii water isolates. Research in Microbiology, 172(1), 103790pt_PT
dc.identifier.doi10.1016/j.resmic.2020.11.001pt_PT
dc.identifier.eid85096587104
dc.identifier.issn0923-2508
dc.identifier.pmid33197514
dc.identifier.urihttp://hdl.handle.net/10400.14/31459
dc.identifier.wos000620952500009
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAssessing the risks associated with environmental antibiotic resistant bacteria: propagation and transmission to humans
dc.relationCentre for Biotechnology and Fine Chemistry
dc.subjectIntrinsic antibiotic resistancept_PT
dc.subjectGentamicin resistancept_PT
dc.subjectArsenite resistancept_PT
dc.subjectUbiquitous bacteriapt_PT
dc.subjectIntegrative and conjugative elements (ICEs)pt_PT
dc.titleEvolution of gentamicin and arsenite resistance acquisition in Ralstonia pickettii water isolatespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssessing the risks associated with environmental antibiotic resistant bacteria: propagation and transmission to humans
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCTA-AMB%2F28196%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.citation.titleResearch in Microbiologypt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVaz-Moreira
person.familyNameManaia
person.givenNameIvone
person.givenNameCélia
person.identifier602434
person.identifier.ciencia-id9E17-544E-800D
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0001-8912-9836
person.identifier.orcid0000-0002-3674-1789
person.identifier.scopus-author-id16044425800
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationa5f14577-0aed-4046-97da-5f4216c7aa07
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