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Advisor(s)
Abstract(s)
A bacterial strain capable of aerobic degradation of
4-fluorocinnamic acid (4-FCA) as the sole source of carbon
and energy was isolated from a biofilm reactor operating for
the treatment of 2-fluorophenol. The organism, designated as
strain S2, was identified by 16S rRNA gene analysis as a
member of the genus Rhodococcus. Strain S2 was able to
mineralize 4-FCA as sole carbon and energy source. In the
presence of a conventional carbon source (sodium acetate
[SA]), growth rate of strain S2 was enhanced from 0.04 to
0.14 h−1 when the culture medium was fed with 0.5 mMof 4-
FCA, and the time for complete removal of 4-FCA decreased
from 216 to 50 h.When grown in SA-supplemented medium,
4-FCA concentrations up to 1 mM did not affect the length of
the lag phase, and for 4-FCA concentrations up to 3 mM,
strain S2 was able to completely remove the target fluorinated
compound. 4-Fluorobenzoate (4-FBA) was transiently formed
in the culture medium, reaching concentrations up to 1.7 mM
when the cultures were supplemented with 3.5mMof 4-FCA.
Trans,trans-muconate was also transiently formed as a metabolic
intermediate. Compounds with molecular mass compatible
with 3-carboxymuconate and 3-oxoadipate were also
detected in the culture medium. Strain S2 was able to mineralize
a range of other haloorganic compounds, including 2-
fluorophenol, to which the biofilm reactor had been exposed.
To our knowledge, this is the first time that mineralization of 4-FCA as the sole carbon source by a single bacterial culture is
reported.
Description
Keywords
4-Fluorocinnamic acid Rhodococcus sp Biodegradation Metabolism
Citation
AMORIM, Catarina L... [et al.] - Mineralization of 4-fluorocinnamic acid by a Rhodococcus strain. Applied Microbiology and Biotechnology. ISSN 1432-0614. Vol. 98, n.º 4 (2014), p. 1893-1905
