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Effect of 1-carbaldehyde-3,4-dimethoxyxanthone on prostate and HPV-18 positive cervical cancer cell lines and on human THP-1 macrophages

2021-06-02Journal article Open access
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dc.contributor.authorMedeiros, Rui
dc.contributor.authorHorta, Bruno
dc.contributor.authorFreitas-Silva, Joana
dc.contributor.authorSilva, Jani
dc.contributor.authorDias, Francisca
dc.contributor.authorSousa, Emília
dc.contributor.authorPinto, Madalena
dc.contributor.authorCerqueira, Fátima
dc.date.accessioned2021-07-08T11:25:07Z
dc.date.available2021-07-08T11:25:07Z
dc.date.issued2021-06-02
dc.description.abstractXanthone derivatives have shown promising antitumor properties, and 1-carbaldehyde-3,4-dimethoxyxanthone (1) has recently emerged as a potent tumor cell growth inhibitor. In this study, its effect was evaluated (MTT viability assay) against a new panel of cancer cells, namely cervical cancer (HeLa), androgen-sensitive (LNCaP) and androgen-independent (PC-3) prostate cancer, and nonsolid tumor derived cancer (Jurkat) cell lines. The effect of xanthone 1 on macrophage functions was also evaluated. The effect of xanthone 1-conditioned THP-1 human macrophage supernatants on the metabolic viability of cervical and prostate cancer cell lines was determined along with its interference with cytokine expression characteristic of M1 profile (IL-1 ≤ β; TNF-α) or M2 profile (IL-10; TGF-β) (PCR and ELISA). Nitric oxide (NO) production by murine RAW264.7 macrophages was quantified by Griess reaction. Xanthone 1 (20 µM) strongly inhibited the metabolic activity of the cell lines and was significantly more active against prostate cell lines compared to HeLa (p < 0.05). Jurkat was the cell most sensitive to the effect of xanthone 1. Compound 1-conditioned IL-4-stimulated THP-1 macrophage supernatants significantly (p < 0.05) inhibited the metabolic activity of HeLa, LNCaP, and PC-3. Xanthone 1 did not significantly affect the expression of cytokines by THP-1 macrophages. The inhibiting effect of compound 1 observed on the production of NO by RAW 264.7 macrophages was moderate. In conclusion, 1-carbaldehyde-3,4-dimethoxyxanthone (1) decreases the metabolic activity of cancer cells and seems to be able to modulate macrophage functions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/molecules26123721pt_PT
dc.identifier.eid85108885389
dc.identifier.issn1420-3049
dc.identifier.pmcPMC8235309
dc.identifier.pmid34207168
dc.identifier.urihttp://hdl.handle.net/10400.14/34105
dc.identifier.wos000666292300001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subject1-carbaldehyde-3,4-dimethoxyxanthonept_PT
dc.subjectAntitumorpt_PT
dc.subjectCervical cancerpt_PT
dc.subjectProstate cancerpt_PT
dc.titleEffect of 1-carbaldehyde-3,4-dimethoxyxanthone on prostate and HPV-18 positive cervical cancer cell lines and on human THP-1 macrophagespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume26pt_PT
person.familyNameMedeiros
person.familyNameGuedes Horta
person.familyNameSilva
person.familyNameDias
person.familyNameSousa
person.familyNamePinto
person.familyNameCerqueira
person.givenNameRui
person.givenNameBruno Miguel
person.givenNameJani
person.givenNameFrancisca
person.givenNameMaria Emília
person.givenNameMadalena
person.givenNameFátima
person.identifier37604
person.identifier812695
person.identifier.ciencia-idC51F-5DBE-9C51
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person.identifier.orcid0000-0002-4993-4467
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person.identifier.orcid0000-0002-4676-1409
person.identifier.orcid0000-0003-4513-4654
person.identifier.ridC-7938-2009
person.identifier.ridD-3814-2017
person.identifier.ridP-1272-2018
person.identifier.ridL-1126-2014
person.identifier.ridL-1835-2014
person.identifier.ridM-7557-2013
person.identifier.scopus-author-id7006241641
person.identifier.scopus-author-id57199792326
person.identifier.scopus-author-id57193868615
person.identifier.scopus-author-id7004889524
person.identifier.scopus-author-id35591287300
person.identifier.scopus-author-id6603346911
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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