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Insights into the protective role of solid lipid nanoparticles on rosmarinic acid bioactivity during exposure to simulated gastrointestinal conditions

2016Journal article Restricted access
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dc.contributor.authorMadureira, Ana Raquel
dc.contributor.authorCampos, Débora A.
dc.contributor.authorOliveira, Ana L.
dc.contributor.authorSarmento, Bruno
dc.contributor.authorPintado, M. E.
dc.contributor.authorGomes, Ana M. P.
dc.date.accessioned2017-10-17T10:05:19Z
dc.date.available2017-10-17T10:05:19Z
dc.date.issued2016
dc.description.abstractThe evaluation of the digestion effects on bioactive solid lipid nanoparticles (SLN) was performed. For this purpose, witepsol and carnauba SLN loaded with rosmarinic acid (RA) were exposed to the simulated gastrointestinal tract (GIT) conditions prevailing in stomach and small intestine. The simulation of intestinal epithelium was made with a dialysis bag and intestinal cell culture lines. Changes on SLN physical properties, RA release and absorption profiles were followed at each step. Combination of digestion pH and enzymes showed a significant effect upon SLN physical properties. Zeta potential values increased at stomach conditions and decreased at small intestine simulation. Also, at intestine, SLN increased their sizes and released 40-60% of RA, maintaining its initial antioxidant activity values. Sustained release of 40% of RA from SLN was also observed in dialysis tube. At CaCo-2 cell line, both types of SLN showed similar absorbed RA % (ca. 30%). Nevertheless, in CaCo-2/HT29x mix cell lines, for carnauba SLN a lower adsorption RA % was observed than for witepsol SLN. Solid lipid nanoparticles protected RA bioactivity (in terms of antioxidant activity) until reaching the intestine. A controlled release of RA from SLN was achieved and a significant absorption was observed at intestinal cells. Overall, SLN produced with witepsol showed a higher stability than carnauba SLN.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMADUREIRA, Ana Raquel; CAMPOS, Débora A.; OLIVEIRA, Ana L.; SARMENTO, Bruno; PINTADO, M. E.; GOMES, Ana M. P. - Insights into the protective role of solid lipid nanoparticles on rosmarinic acid bioactivity during exposure to simulated gastrointestinal conditions. Colloids and Surfaces B: Biointerfaces. ISSN 0927-7765. Vol. 139 (2016), p. 277–284pt_PT
dc.identifier.doi10.1016/j.colsurfb.2015.11.039pt_PT
dc.identifier.eid84952896924
dc.identifier.pmid26766625
dc.identifier.urihttp://hdl.handle.net/10400.14/23142
dc.identifier.wos000389687900034
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationSAESCTN-PIIC&DT/2011pt_PT
dc.relationStrategic Project - LA 16 - 2013-2014
dc.relationStrategic Project - LA 2 - 2013-2014
dc.relationEVALUATION OF STABILITY AND TOXICITY OF NANOPARTICLES AS DELIVERY SYSTEMS OF BIOACTIVE SUBSTANCES IN DAIRY PRODUCTS
dc.subjectAntioxidantspt_PT
dc.subjectBiomimetic Materialspt_PT
dc.subjectCaco-2 Cellspt_PT
dc.subjectCinnamatespt_PT
dc.subjectDelayed-Action Preparationspt_PT
dc.subjectDepsidespt_PT
dc.subjectDialysispt_PT
dc.subjectDrug Compoundingpt_PT
dc.subjectDrug Liberationpt_PT
dc.subjectGastric Juicept_PT
dc.subjectHT29 Cellspt_PT
dc.subjectHumanspt_PT
dc.subjectHydrogen-Ion Concentrationpt_PT
dc.subjectIntestinal Absorptionpt_PT
dc.subjectIntestine, Smallpt_PT
dc.subjectModels, Biologicalpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectPancreatinpt_PT
dc.subjectParticle Sizept_PT
dc.subjectPepsin Apt_PT
dc.subjectStomachpt_PT
dc.subjectTriglyceridespt_PT
dc.subjectWaxespt_PT
dc.titleInsights into the protective role of solid lipid nanoparticles on rosmarinic acid bioactivity during exposure to simulated gastrointestinal conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 16 - 2013-2014
oaire.awardTitleStrategic Project - LA 2 - 2013-2014
oaire.awardTitleEVALUATION OF STABILITY AND TOXICITY OF NANOPARTICLES AS DELIVERY SYSTEMS OF BIOACTIVE SUBSTANCES IN DAIRY PRODUCTS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-ALI%2F117808%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FLA0002%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F71391%2F2010/PT
oaire.citation.titleColloids and Surfaces B: Biointerfacespt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamFARH
person.familyNameMadureira
person.familyNameCampos
person.familyNameOliveira
person.familyNameSarmento
person.familyNamePintado
person.familyNameGomes
person.givenNameRaquel
person.givenNameDébora
person.givenNameAna
person.givenNameBruno
person.givenNameMaria Manuela
person.givenNameAna Maria
person.identifierM-4659-2013
person.identifierD-8675-2016
person.identifier1415634
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person.identifier.ciencia-id1C14-25F8-CC07
person.identifier.ciencia-id1A16-FC5B-3468
person.identifier.ciencia-id4713-31C8-A356
person.identifier.ciencia-idE816-B6B0-06A5
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-idAA12-BFC1-B2E3
person.identifier.orcid0000-0001-6596-1927
person.identifier.orcid0000-0003-0174-6640
person.identifier.orcid0000-0001-8012-4203
person.identifier.orcid0000-0001-5763-7553
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0001-7883-2446
person.identifier.ridM-4659-2013
person.identifier.ridJ-3404-2013
person.identifier.ridJ-6265-2013
person.identifier.ridF-5696-2013
person.identifier.ridB-9944-2013
person.identifier.scopus-author-id8384097900
person.identifier.scopus-author-id55250923400
person.identifier.scopus-author-id55937245800
person.identifier.scopus-author-id57194334236
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id57190668768
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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