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1,3-Propanediol production in a two-step process fermentation from renewable feedstock

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dc.contributor.authorMendes, Filipa Soares
dc.contributor.authorGonzález-Pajuelo, Maria
dc.contributor.authorCordier, Hélène
dc.contributor.authorFrançois, Jean M.
dc.contributor.authorVasconcelos, Isabel
dc.date.accessioned2012-01-18T17:57:10Z
dc.date.available2012-01-18T17:57:10Z
dc.date.issued2011
dc.description.abstractIn this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>200 g l−1) glucose concentrations. The second step, carried out in the same bioreactor, was performed by the engineered strain Clostridium acetobutylicum DG1 (pSPD5) that converts glycerol to 1,3-propanediol. This two-step strategy led to a flexible process, resulting in a 1,3-propanediol production and yield that depended on the initial sugar concentration. Below 56.2 g l−1 of sugar concentration, cultivation on molasses or glucose showed no significant differences. However, at higher molasses concentrations, glycerol initially produced by yeast could not be totally converted into 1,3-propanediol by C. acetobutylicum and a lower 1,3-propanediol overall yield was observed. In our hand, the best results were obtained with an initial glucose concentration of 103 g l−1, leading to a final 1,3-propanediol concentration of 25.5 g l−1, a productivity of 0.16 g l−1 h−1 and 1,3-propanediol yields of 0.56 g g−1 glycerol and 0.24 g g−1 sugar, which is the highest value reported for a two-step process. For an initial sugar concentration (from molasses) of 56.2 g l−1, 27.4 g l−1 of glycerol were produced, leading to 14.6 g l−1 of 1.3-propanediol and similar values of productivity, 0.15 g l−1 h−1, and overall yield, 0.26 g g−1 sugar.por
dc.description.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.citationMENDES, Filipa Soares... [et al.] - 1,3-Propanediol production in a two-step process fermentation from renewable feedstock. Applied Microbiology and Biotechnology. ISSN 1432-0614. Vol. 92, n.º 3 (2011), p. 519-527por
dc.identifier.doi10.1007/s00253-011-3369-1
dc.identifier.eissn1432-0614
dc.identifier.issn0175-7598
dc.identifier.urihttp://hdl.handle.net/10400.14/7509
dc.identifier.wosWOS:000295673800009
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relationQLRT-1999-01364
dc.subjectMolassespor
dc.subjectGlycerolpor
dc.subject1,3-propanediolpor
dc.subjectTwo-step fermentationpor
dc.subjectSaccharomyces cerevisiaepor
dc.subjectClostridium acetobutylicumpor
dc.title1,3-Propanediol production in a two-step process fermentation from renewable feedstockpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F8337%2F2002/PT
oaire.citation.endPage527
oaire.citation.issue3
oaire.citation.startPage519
oaire.citation.titleApplied Microbiology and Biotechnology
oaire.citation.volume92
oaire.fundingStreamSFRH
person.familyNameCordier
person.familyNameVasconcelos
person.givenNameHelene
person.givenNameIsabel
person.identifier.ciencia-id851B-41B9-4746
person.identifier.orcid0000-0003-1634-621X
person.identifier.orcid0000-0002-0565-3516
person.identifier.ridN-3870-2013
person.identifier.scopus-author-id7003767794
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication1522d7c2-3a42-4305-af89-e4678f12301e
relation.isAuthorOfPublication251ce926-a408-483f-a3df-acebe6631477
relation.isAuthorOfPublication.latestForDiscovery251ce926-a408-483f-a3df-acebe6631477
relation.isProjectOfPublicationf7f9b53c-7cc6-47aa-bfe2-1f0dea871b91
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