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Development and validation of novel PCR primers for identification of plasmid-mediated colistin resistance (mcr) genes in various environmental settings

2022-03-05Journal article Open access
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dc.contributor.authorGorecki, Adrian
dc.contributor.authorMusialowski, Marcin
dc.contributor.authorWolacewicz, Mikolaj
dc.contributor.authorDecewicz, Przemyslaw
dc.contributor.authorFerreira, Catarina
dc.contributor.authorVejmelkova, Dana
dc.contributor.authorGrzesiuk, Malgorzata
dc.contributor.authorManaia, Célia M.
dc.contributor.authorBartacek, Jan
dc.contributor.authorDziewit, Lukasz
dc.date.accessioned2021-12-21T18:59:09Z
dc.date.available2021-12-21T18:59:09Z
dc.date.issued2022-03-05
dc.description.abstractAntibiotic resistance is considered one of the biggest threats to public health and has become a major concern for governments and international organizations. Combating this problem starts with improving global surveillance of antibiotic resistance genes (ARGs) and applying standardized protocols, both in a clinical and environmental context, in agreement with the One Health approach. Exceptional efforts should be directed to controlling ARGs conferring resistance to Critically Important Antimicrobials (CIA). In this study, a systematic literature review to synthesize data on the identification of mcr genes using a PCR technique was performed. Additionally, a novel set of PCR primers for mcr-1 – mcr-9 genes detection was proposed. The developed primers were in silico and experimentally validated by comparison with mcr-specific PCR primers reported in the literature. This validation, besides being a proof-of-concept for primers’ usefulness, provided insight into the distribution of mcr genes in municipal wastewater, clay and river sediments, glacier moraine, manure, seagulls and auks feces and daphnids from four countries. This analysis proved that commonly used primers may deliver false results, and some mcr genes may be overlooked in tested samples. Newly-developed PCR primers turned out to be relevant for the screening of mcr genes in various environments.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jhazmat.2021.127936pt_PT
dc.identifier.eid85120827539
dc.identifier.issn0304-3894
dc.identifier.pmid34883371
dc.identifier.urihttp://hdl.handle.net/10400.14/36240
dc.identifier.wos000734370000005
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntibiotic resistance genept_PT
dc.subjectColistinpt_PT
dc.subjectMcr genept_PT
dc.subjectOne healthpt_PT
dc.subjectPCR primerpt_PT
dc.titleDevelopment and validation of novel PCR primers for identification of plasmid-mediated colistin resistance (mcr) genes in various environmental settingspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleJournal of Hazardous Materialspt_PT
oaire.citation.volume425pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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