Repository logo
 
Publication

Short-term responses of soil microbial communities to changes in air temperature, soil moisture and UV radiation

2022-05-10Journal article Open access
Simple item page
dc.contributor.authorSilva, Isabel
dc.contributor.authorAlves, Marta
dc.contributor.authorMalheiro, Catarina
dc.contributor.authorSilva, Ana Rita R.
dc.contributor.authorLoureiro, Susana
dc.contributor.authorHenriques, Isabel
dc.contributor.authorGonzález-Alcaraz, M. Nazaret
dc.date.accessioned2022-06-06T15:34:30Z
dc.date.available2022-06-06T15:34:30Z
dc.date.issued2022-05-10
dc.description.abstractWe analyzed the effects on a soil microbial community of short-term alterations in air temperature, soil moisture and ultraviolet radiation and assessed the role of invertebrates (species Enchytraeus crypticus) in modulating the community’s response to these factors. The reference soil, Lufa 2.2, was incubated for 48 h, with and without invertebrates, under the following conditions: standard (20 °C + 50% water holding capacity (WHC)); increased air temperature (15 °C–25 °C or 20 °C–30 °C + 50% WHC); flood (20 °C + 75% WHC); drought (20 °C + 25% WHC); and ultraviolet radiation (UV) (20 °C + 50% WHC + UV). BIOLOG EcoPlates and 16S rDNA sequencing (Illumina) were used to assess the microbial community’s physiological profile and the bacterial community’s structure, respectively. The bacterial abundance (estimated by 16S rDNA qPCR) did not change. Most of the conditions led to an increase in microbial activity and a decrease in diversity. The structure of the bacterial community was particularly affected by higher air temperatures (20 °C–30 °C, without E. crypticus) and floods (with E. crypticus). Effects were observed at the class, genera and OTU levels. The presence of invertebrates mostly resulted in the attenuation of the observed effects, highlighting the importance of considering microbiome–invertebrate interactions. Considering future climate changes, the effects described here raise concern. This study provides fundamental knowledge to develop effective strategies to mitigate these negative outcomes. However, long-term studies integrating biotic and abiotic factors are needed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/genes13050850pt_PT
dc.identifier.eid85130402554
dc.identifier.issn2073-4425
dc.identifier.pmcPMC9142034
dc.identifier.pmid35627235
dc.identifier.urihttp://hdl.handle.net/10400.14/37827
dc.identifier.wos000802606400001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBacterial diversitypt_PT
dc.subjectDroughtpt_PT
dc.subjectFloodpt_PT
dc.subjectIncreased temperaturept_PT
dc.subjectMetagenomicspt_PT
dc.subjectMicrobial activitypt_PT
dc.subjectQuantitative PCRpt_PT
dc.subjectSoil invertebratespt_PT
dc.subjectSoil microbiomept_PT
dc.subjectUV exposurept_PT
dc.titleShort-term responses of soil microbial communities to changes in air temperature, soil moisture and UV radiationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue5pt_PT
oaire.citation.titleGenespt_PT
oaire.citation.volume13pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
44054812.pdf
Size:
20.58 MB
Format:
Adobe Portable Document Format
Download

Collections

CBQF - Contribuições em Revistas Científicas / Contribution to Journals