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Exploring the expression of defence-related genes in Actinidia spp. after infection with Pseudomonas syringae pv. actinidiae and pv. actinidifoliorum: first steps

2019Journal article Open access
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dc.contributor.authorSilva, M. Nunes da
dc.contributor.authorMachado, J.
dc.contributor.authorBalestra, G. M.
dc.contributor.authorMazzaglia, A.
dc.contributor.authorVasconcelos, M. W.
dc.contributor.authorCarvalho, S. M. P.
dc.date.accessioned2019-10-17T15:20:28Z
dc.date.available2019-10-17T15:20:28Z
dc.date.issued2019
dc.description.abstractKiwifruit bacterial canker (KBC), caused by Pseudomonas syringae pv. actinidiae (PSA), is currently the most destructive disease of kiwifruit worldwide. Conversely, a closely related bacterial strain, P. syringae pv. actinidifoliorum (PFM), only causes necrotic spots and has not been associated with plant mortality. Moreover, there is some evidence on the higher susceptibility of the Actinidia chinensis var. deliciosa kiwifruit species to KBC, compared with A. arguta, but the reasons behind it are still largely unknown. In this work, micropropagated plants of Actinidia chinensis var. deliciosa 'Hayward' and A. arguta var. arguta 'Ken's Red' were inoculated with PSA or with PFM (10(7) CFUs mL(-1)). Disease development was monitored 1, 2 and 5 days post inoculation (dpi) through the determination colony forming units (CFUs) and the expression analysis of six plant defence-related genes (APX, CAT, SOD, LOX1, SAM and TLP1). At 5 dpi, CFUs in plant tissues inoculated with PSA and PFM were, respectively, 17.4-fold and 2.8-fold higher in A. chinensis compared with A. arguta. Expression of antioxidant enzyme-related genes was very distinct between the two kiwifruit species: SOD expression was drastically increased in A. chinensis (up to 2.1-fold, 5 dpi), whereas in A. arguta CAT was the most upregulated gene (up to 1.7-fold, 2 dpi). LOX1, involved in jasmonic acid biosynthesis, was upregulated in both species, however reaching the highest values at 2 dpi in A. chinensis (2.2 fold) and 1 dpi in A. arguta (1.9-fold). It is concluded that A. arguta is much more tolerant to PSA than A. chinensis and that the molecular mechanisms between the two kiwifruit species involve specific defence pathways being triggered at distinct moments after plant infection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNunes da Silva, M., Machado, J., Balestra, G. M., Mazzaglia, A., … Vasconcelos, M. W. (2019). Exploring the expression of defence-related genes in Actinidia spp. after infection with Pseudomonas syringae pv. actinidiae and pv. actinidifoliorum: first steps. European Journal of Horticultural Science, 84(4), 206–212. https://doi.org/10.17660/ejhs.2019/84.4.2pt_PT
dc.identifier.doi10.17660/eJHS.2019/84.4.2pt_PT
dc.identifier.eid85073451224
dc.identifier.issn1611-4426
dc.identifier.urihttp://hdl.handle.net/10400.14/28415
dc.identifier.wos000484764200002
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInternational Society for Horticultural Sciencept_PT
dc.relationKiwi Bacterial Canker: Exploring Tolerance Mechanisms and Novel Control Techniques
dc.subjectAntioxidant enzymespt_PT
dc.subjectBacterial cankerpt_PT
dc.subjectKiwifruitpt_PT
dc.subjectPFMpt_PT
dc.subjectPSApt_PT
dc.subjectSusceptibilitypt_PT
dc.titleExploring the expression of defence-related genes in Actinidia spp. after infection with Pseudomonas syringae pv. actinidiae and pv. actinidifoliorum: first stepspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleKiwi Bacterial Canker: Exploring Tolerance Mechanisms and Novel Control Techniques
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-PRO%2F6156%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F99853%2F2014/PT
oaire.citation.endPage212
oaire.citation.issue4
oaire.citation.startPage206
oaire.citation.titleEuropean Journal of Horticultural Sciencept_PT
oaire.citation.volume84
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameNunes da Silva
person.familyNameBALESTRA
person.familyNameMazzaglia
person.familyNameVasconcelos
person.familyNameCarvalho
person.givenNameMarta
person.givenNameGiorgio
person.givenNameAngelo
person.givenNameMarta
person.givenNameSusana M.P.
person.identifier1023870
person.identifier1024328
person.identifier74696
person.identifier194747
person.identifier.ciencia-id3C1B-7E7B-F695
person.identifier.ciencia-id5E16-859A-37A4
person.identifier.orcid0000-0001-8228-0576
person.identifier.orcid0000-0002-0822-4506
person.identifier.orcid0000-0002-1295-2565
person.identifier.orcid0000-0002-5110-7006
person.identifier.orcid0000-0001-7157-1079
person.identifier.ridI-4336-2012
person.identifier.ridI-8166-2013
person.identifier.ridI-7184-2013
person.identifier.scopus-author-id55576995600
person.identifier.scopus-author-id12797822900
person.identifier.scopus-author-id6603557560
person.identifier.scopus-author-id14627797100
person.identifier.scopus-author-id7005619826
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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