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Sequential injection system for the enzymatic determination of ethanol in alcoholic beverages with in-line dilution

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dc.contributor.authorPais, Teresa F. M.
dc.contributor.authorVidigal, Susana S. M. P.
dc.contributor.authorTóth, Ildikó V.
dc.contributor.authorRangel, António O. S. S.
dc.date.accessioned2013-01-28T13:23:48Z
dc.date.available2013-01-28T13:23:48Z
dc.date.issued2013
dc.description.abstractA sequential injection system for the enzymatic determination of ethanol in wines with spectrophotometric detection was developed. The determination was based on the enzymatic reaction involving ethanol and NAD(+) in the presence of alcohol dehydrogenase. A gas-diffusion unit was introduced in the system to perform an in-line dilution. It was possible to operate the system with a reagent consumption of 1.3 mg of NAD(+), 0.45 U of ADH, and 32 mu L of sample, per assay with a determination rate of 21 h(-1). The results obtained from the analysis of wine samples were statistically equivalent to those obtained by the reference method, with good repeatability; the relative standard deviation (n = 10) was <1% with a LOQ of 0.3% (v/v). (C) 2012 Elsevier Ltd. All rights reserved.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationPAIS, Teresa F. M... [et al.] - Sequential injection system for the enzymatic determination of ethanol in alcoholic beverages with in-line dilution. Food Control. ISSN 0956-7135. Vol. 30, n.º 2 (2013), p. 616-620por
dc.identifier.doi10.1016/j.foodcont.2012.08.013
dc.identifier.eissn1873-7129
dc.identifier.issn0956-7135
dc.identifier.urihttp://hdl.handle.net/10400.14/9986
dc.identifier.wosWOS:000313535500042
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationStrategic Project - LA 16 - 2011-2012
dc.relationNEW APPROACHES FOR REAGENT BASED BIOCHEMICAL ASSAYS USING ADVANCED FLOW TECHNIQUES
dc.relationDEVELOPMENT OF AUTOMATIC LOW PRESSURE CHROMATOGRAPHIC FLOW SYSTEMS FOR WINE ANALYSIS, BASED ON MONOLITHIC COLUMNS AND MINIATURIZED DETECTORS
dc.relationStrategic Project - LA 6 - 2011-2012
dc.subjectSequential injection analysis systempor
dc.subjectEthanol determinationpor
dc.subjectWinepor
dc.subjectAlcohol dehydrogenasepor
dc.subjectGas-diffusion unitpor
dc.subjectSpectrophotometrypor
dc.titleSequential injection system for the enzymatic determination of ethanol in alcoholic beverages with in-line dilutionpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardTitleNEW APPROACHES FOR REAGENT BASED BIOCHEMICAL ASSAYS USING ADVANCED FLOW TECHNIQUES
oaire.awardTitleDEVELOPMENT OF AUTOMATIC LOW PRESSURE CHROMATOGRAPHIC FLOW SYSTEMS FOR WINE ANALYSIS, BASED ON MONOLITHIC COLUMNS AND MINIATURIZED DETECTORS
oaire.awardTitleStrategic Project - LA 6 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F23040%2F2005/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F78705%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FEQB%2FLA0006%2F2011/PT
oaire.citation.endPage620
oaire.citation.issue2
oaire.citation.startPage616
oaire.citation.titleFood Control
oaire.citation.volume30
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamFARH
oaire.fundingStreamFARH
oaire.fundingStream6820 - DCRRNI ID
person.familyNameVidigal
person.familyNameTóth
person.familyNameSantos Silva Rangel
person.givenNameSusana
person.givenNameIldikó
person.givenNameAntónio Osmaro
person.identifier.ciencia-id2919-B77C-11C0
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0002-0213-063X
person.identifier.orcid0000-0002-7342-1063
person.identifier.orcid0000-0002-6486-8947
person.identifier.ridM-4661-2013
person.identifier.ridG-1733-2010
person.identifier.scopus-author-id11739488200
person.identifier.scopus-author-id35512332800
person.identifier.scopus-author-id7004827387
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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