Publicação
Characterisation of ‘Starking’ apple polyphenoloxidase
| dc.contributor.author | Rocha, Ada M. C. N. | |
| dc.contributor.author | Cano, M. Pilar | |
| dc.contributor.author | Galeazzi, Maria A. M. | |
| dc.date.accessioned | 2011-10-20T17:22:37Z | |
| dc.date.available | 2011-10-20T17:22:37Z | |
| dc.date.issued | 1998 | |
| dc.description.abstract | Experiments were performed to optimise the extraction conditions of “StarkingÏ apple fruit polyphenoloxidase (PPO), to evaluate the affinity and speciÐcity towards several substrates and to study the stability of enzyme extracts from apple samples stored under di†erent conditions. Sodium phosphate bu†er (0É2 M, pH 6É5) plus 0É25% Triton X and 1% or 2% PVPP was found to be the most efficient extraction mediu10m0. Chlorogenic acid, dopamine and 4- methylcatechol showed similar speciÐcity towards PPO, and chlorogenic acid was found to be the best substrate for the enzyme. Enzyme extracts from frozen cut apple stored at [4¡C, and extracts from lyophilised apple samples stored at 4¡C were more stable than extracts obtained from fresh-cut or acetone powder samples | por |
| dc.identifier.citation | ROCHA, Ada M. C. N.; CANO M. Pilar; GALEAZZI, Maria A. M. - Characterisation of ‘Starking’ apple polyphenoloxidase. Journal of the Science of Food and Agriculture. ISSN 0022-5142. Vol. 77 (1998), p. 527-534 | por |
| dc.identifier.doi | 10.1002/(SICI)1097-0010(199808)77:4<527::AID-JSFA76>3.0.CO;2-E | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/6545 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Wiley | por |
| dc.subject | Acetone powder | por |
| dc.subject | Apple | por |
| dc.subject | Freezing | por |
| dc.subject | Lyophilisation | por |
| dc.subject | StarkingÏ | por |
| dc.subject | Storage | por |
| dc.title | Characterisation of ‘Starking’ apple polyphenoloxidase | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Rocha | |
| person.givenName | Ada Margarida Correia Nunes Da | |
| person.identifier.ciencia-id | 241C-6367-5DDE | |
| person.identifier.orcid | 0000-0003-3505-4538 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | ef58e6b5-0f87-465e-bc54-9a5c7c5eb0d0 | |
| relation.isAuthorOfPublication.latestForDiscovery | ef58e6b5-0f87-465e-bc54-9a5c7c5eb0d0 |