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Tumor growth suppression induced by biomimetic silk fibroin hydrogels

2016Journal article Open access
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dc.contributor.authorYan, Le-Ping
dc.contributor.authorSilva-Correia, Joana
dc.contributor.authorRibeiro, Viviana P.
dc.contributor.authorMiranda-Gonçalves, Vera
dc.contributor.authorCorreia, Cristina
dc.contributor.authorMorais, Alain da Silva
dc.contributor.authorSousa, Rui A.
dc.contributor.authorReis, Rui M.
dc.contributor.authorOliveira, Ana L.
dc.contributor.authorOliveira, Joaquim M.
dc.contributor.authorReis, Rui L.
dc.date.accessioned2017-10-16T15:26:39Z
dc.date.available2017-10-16T15:26:39Z
dc.date.issued2016
dc.description.abstractProtein-based hydrogels with distinct conformations which enable encapsulation or differentiation of cells are of great interest in 3D cancer research models. Conformational changes may cause macroscopic shifts in the hydrogels, allowing for its use as biosensors and drug carriers. In depth knowledge on how 3D conformational changes in proteins may affect cell fate and tumor formation is required. Thus, this study reports an enzymatically crosslinked silk fibroin (SF) hydrogel system that can undergo intrinsic conformation changes from random coil to β-sheet conformation. In random coil status, the SF hydrogels are transparent, elastic, and present ionic strength and pH stimuli-responses. The random coil hydrogels become β-sheet conformation after 10 days in vitro incubation and 14 days in vivo subcutaneous implantation in rat. When encapsulated with ATDC-5 cells, the random coil SF hydrogel promotes cell survival up to 7 days, whereas the subsequent β-sheet transition induces cell apoptosis in vitro. HeLa cells are further incorporated in SF hydrogels and the constructs are investigated in vitro and in an in vivo chick chorioallantoic membrane model for tumor formation. In vivo, Angiogenesis and tumor formation are suppressed in SF hydrogels. Therefore, these hydrogels provide new insights for cancer research and uses of biomaterials.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationYAN, Le-Ping; SILVA-CORREIA, Joana; RIBEIRO, Viviana P.; MIRANDA-GONÇALVES, Vera; CORREIA, Cristina; MORAIS, Alain da Silva; SOUSA, Rui A.; REIS, Rui M.; OLIVEIRA, Ana L.; OLIVEIRA, Joaquim M.; REIS, Rui L. - Tumor growth suppression induced by biomimetic silk fibroin hydrogels. Scientific Reports. ISSN 2045-2322. Vol. 6 (2016), 11 p. (31037)pt_PT
dc.identifier.doi10.1038/srep31037pt_PT
dc.identifier.eid84982730530
dc.identifier.pmcPMC4971568
dc.identifier.pmid27485515
dc.identifier.urihttp://hdl.handle.net/10400.14/23117
dc.identifier.wos000380646900001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationFP7/2007–2013pt_PT
dc.relationREGPOT-CT2012-316331-POLARISpt_PT
dc.relationTISSUE2TISSUE-Using Textile Technologies to Develop Innovative 3D Architectures to Be Applied in Bone Tissue Engineering
dc.relationIF/00423/2012pt_PT
dc.relationNATURE-INSPIRED SILK/POLYAMINE-CALCIUM PHOSPHATE COMPOSITES WITH CONTROLLED SURFACE TOPOGRAPHY VIA BIO-INDUCED DEPOSITION OF MULTIFUNCTIONAL SILICON-SUBSTITUTED CALCIUM PHOSPHATE FOR BONE TISSUE ENGINEERING APPLICATIONS
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleTumor growth suppression induced by biomimetic silk fibroin hydrogelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTISSUE2TISSUE-Using Textile Technologies to Develop Innovative 3D Architectures to Be Applied in Bone Tissue Engineering
oaire.awardTitleNATURE-INSPIRED SILK/POLYAMINE-CALCIUM PHOSPHATE COMPOSITES WITH CONTROLLED SURFACE TOPOGRAPHY VIA BIO-INDUCED DEPOSITION OF MULTIFUNCTIONAL SILICON-SUBSTITUTED CALCIUM PHOSPHATE FOR BONE TISSUE ENGINEERING APPLICATIONS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FCTM-BPC%2F115977%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FCTM%2F105703%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F64717%2F2009/PT
oaire.citation.titleScientific Reportspt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
person.familyNameYan
person.familyNameSilva Correia
person.familyNameRibeiro
person.familyNameMiranda-Gonçalves
person.familyNameCorreia
person.familyNameda Silva Morais
person.familyNameSousa
person.familyNameReis
person.familyNameOliveira
person.familyNameOliveira
person.familyNameReis
person.givenNameLeping
person.givenNameJoana
person.givenNameDr. Viviana
person.givenNameVera
person.givenNameCristina
person.givenNameAlain
person.givenNameRui
person.givenNameRui
person.givenNameAna
person.givenNameJoaquim Miguel
person.givenNameRui L.
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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