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Modulation of the uptake of critical nutrients by breast cancer cells by lactate: Impact on cell survival, proliferation and migration

2016Journal article Restricted access
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dc.contributor.authorGuedes, Marta
dc.contributor.authorAraújo, João R.
dc.contributor.authorCorreia-Branco, Ana
dc.contributor.authorGregório, Inês
dc.contributor.authorMartel, Fátima
dc.contributor.authorKeating, Elisa
dc.date.accessioned2017-10-13T17:30:28Z
dc.date.available2017-10-13T17:30:28Z
dc.date.issued2016
dc.description.abstractThis work aimed to characterize the uptake of folate and glucose by breast cancer cells and to study the effect of lactate upon the transport of these nutrients and upon cell viability, proliferation and migration capacity. Data obtained showed that: a) MCF7 cells uptake (3)H-folic acid ((3)H-FA) at physiological but not at acidic pH; b) T47D cells accumulate (3)H-FA and (14)C-5-methyltetrahydrofolate ((14)C-5-MTHF) more efficiently at acidic than at physiological pH; c) (3)H-deoxyglucose ((3)H-DG) uptake by T47D cells is sodium-independent, inhibited by cytochalasin B (CYT B) and stimulated by insulin. Regarding the effect of lactate, in T47D cells, acute (26 min) and chronic (24 h) exposure to lactic acid (LA) stimulated (3)H-FA uptake. Acute exposure to LA also stimulated (3)H-DG uptake and chronic exposure to LA significantly stimulated T47D cell migratory capacity. In conclusion, the transport of folates is strikingly different in two phenotypically similar breast cancer cell lines: MCF7 and T47D cells. Additionally, lactate seems to act as a signaling molecule which increases the uptake of nutrients and promotes the migration capacity of T47D cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGUEDES, Marta; ARAÚJO, João R.; CORREIA-BRANCO, Ana; GREGÓRIO, Inês; MARTEL, Fátima; KEATING, Elisa - Modulation of the uptake of critical nutrients by breast cancer cells by lactate: Impact on cell survival, proliferation and migration. Experimental Cell Research. ISSN 0014-4827. Vol. 341, n.º 2 (2016), p. 111-122pt_PT
dc.identifier.doi10.1016/j.yexcr.2016.01.008pt_PT
dc.identifier.eid84958924114
dc.identifier.pmid26794902
dc.identifier.urihttp://hdl.handle.net/10400.14/23080
dc.identifier.wos000371446700001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNORTE-07–0124-FEDER-000052pt_PT
dc.subjectBiological Transportpt_PT
dc.subjectBreast Neoplasmspt_PT
dc.subjectCell Line, Tumorpt_PT
dc.subjectCell Movementpt_PT
dc.subjectCell Proliferationpt_PT
dc.subjectCell Survivalpt_PT
dc.subjectFemalept_PT
dc.subjectFolic Acidpt_PT
dc.subjectGlucosept_PT
dc.subjectHumanspt_PT
dc.subjectLactic Acidpt_PT
dc.titleModulation of the uptake of critical nutrients by breast cancer cells by lactate: Impact on cell survival, proliferation and migrationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-OSM%2F102239%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F40170%2F2007/PT
oaire.citation.endPage122
oaire.citation.issue2
oaire.citation.startPage111
oaire.citation.titleExperimental Cell Researchpt_PT
oaire.citation.volume341
oaire.fundingStream5876-PPCDTI
oaire.fundingStreamSFRH
person.familyNameGuedes
person.familyNameMartel
person.familyNameKeating
person.givenNameMarta
person.givenNameFátima
person.givenNameElisa
person.identifier517090
person.identifier.ciencia-id5C14-37B4-FDCA
person.identifier.ciencia-id2E1C-FAA4-A661
person.identifier.orcid0000-0001-8486-4380
person.identifier.orcid0000-0002-0525-3416
person.identifier.orcid0000-0002-3904-9907
person.identifier.scopus-author-id7005164101
person.identifier.scopus-author-id35612071300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationb8800d60-d712-4d4c-8619-4a33ca095ca6
relation.isAuthorOfPublication66ab6fb4-ef6c-43e6-876e-22fdf8a479e2
relation.isAuthorOfPublication.latestForDiscovery9a9dc232-99b1-4f8d-aec9-1c8872a800fb
relation.isProjectOfPublication82bfad98-f9a5-45e1-ac50-a5deecbd835b
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