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Biological protein precipitation: a green process for the extraction of cucumisin from melon (Cucumis melo L. inodorus) by-products

2021Journal article Open access
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dc.contributor.authorGómez-García, Ricardo
dc.contributor.authorCampos, Débora A.
dc.contributor.authorAguilar, Cristóbal N.
dc.contributor.authorMadureira, Ana R.
dc.contributor.authorPintado, Manuela
dc.date.accessioned2021-02-10T15:32:03Z
dc.date.available2021-02-10T15:32:03Z
dc.date.issued2021
dc.description.abstractCucumisin (CUC) from industrial melon by-products was separated for the first time through biological precipitation using carrageenan (CRG). This approach could represent a cost-effective and environmentally friendly process for the industries, avoiding the use of expensive equipment and toxic salts or solvents, such as butanol and ethanol. In this study, biological precipitation of proteins from melon by-products using CRG was studied and compared with conventional precipitation with ammonium sulphate. Different methods were applied for the identification and characterization of isolated proteins, including SDS-PAGE gel, FPLC and proteolytic activity assays. The isolated CUC confirmed a molecular weight of 68 kDa and showed highly stable proteolytic (PA) and milk-clotting (MCA) activities in a wide range of CaCl2 (20 to 60 mM), pH (5 to 7) and temperatures (30 to 85 ºC). Melon peel extract demonstrated to possess significant PA (4.24 U/mg protein) and MCA (191.50 MCU/mg protein), but such values were increased by ammonium sulphate precipitation (1.60 and 2.06-folds, respectively), and specially a noticeable increment was observed by biological precipitation with 2.11 and 17.65-folds, respectively, demonstrating the capability to be an effective strategy to isolate and purify CUC, allowing a yield of 0.17 g CUC/100 g of by-products and keeping its biological properties.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGómez-García, R., Campos, D. A., Aguilar, C. N., Madureira, A. R., Pintado, M. (2021). Biological protein precipitation: a green process for the extraction of cucumisin from melon (Cucumis melo L. inodorus) by-products. Food Hydrocolloidspt_PT
dc.identifier.doi10.1016/j.foodhyd.2021.106650pt_PT
dc.identifier.eid85101311182
dc.identifier.issn0268-005X
dc.identifier.urihttp://hdl.handle.net/10400.14/32000
dc.identifier.wos000632991300004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-0145-FEDER-016403pt_PT
dc.relationLISBOA-01-0145-FEDER-016403pt_PT
dc.subjectBiological precipitationpt_PT
dc.subjectCucumisinpt_PT
dc.subjectMelon by-productspt_PT
dc.subjectMilk-clotting and proteolytic enzymept_PT
dc.subjectPolyelectrolytept_PT
dc.titleBiological protein precipitation: a green process for the extraction of cucumisin from melon (Cucumis melo L. inodorus) by-productspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/SAICTPAC%2F0040%2F2015/PT
oaire.citation.titleFood Hydrocolloidspt_PT
oaire.fundingStream9471 - RIDTI
person.familyNameCampos
person.familyNameAguilar
person.familyNameMadureira
person.familyNamePintado
person.givenNameDébora
person.givenNameCristobal N.
person.givenNameRaquel
person.givenNameMaria Manuela
person.identifierD-8675-2016
person.identifierM-4659-2013
person.identifier456608
person.identifier.ciencia-id1A16-FC5B-3468
person.identifier.ciencia-id1C14-25F8-CC07
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-0174-6640
person.identifier.orcid0000-0001-5867-8672
person.identifier.orcid0000-0001-6596-1927
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridF-2725-2013
person.identifier.ridM-4659-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id55250923400
person.identifier.scopus-author-id7102461199
person.identifier.scopus-author-id8384097900
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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