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Valorization of spent sugarcane fermentation broth as a source of phenolic compounds

dc.contributor.authorOliveira, Carla Maria
dc.contributor.authorHorta, Bruno
dc.contributor.authorLeal, Tânia
dc.contributor.authorPintado, Manuela
dc.contributor.authorOliveira, Catarina S. S.
dc.date.accessioned2022-07-20T16:25:22Z
dc.date.available2022-07-20T16:25:22Z
dc.date.issued2022-07-08
dc.description.abstractA methodology based on a solid phase extraction (SPE) was optimized for the recovery of phenolic compounds from the spent fermentation broth generated from Biofene® (trans-β-farnesene) production. For this purpose, two resins (XAD-2 and HP-20) and three desorption solutions (water, 50/50 ethanol/water, and ethanol) were tested. The most efficient resin revealed to be the HP-20, using ethanol as desorption solution, reaching an overall total phenolic compound recovery of ca. 80% when 6 BV (bed volume) of both feed and ethanol were applied. The optimization of the resin’s process cycle pointed to 15 BV feed to be treated per cycle and using the same volume of ethanol in the desorption step, with no need for an extra resin regeneration step, stably yielding 48% total phenolic compound recovery from the spent broth for at least 4 cycles, translating into 60 BV of feed being treated per BV of resin, and with the resin being still perfectly active. The extract was characterized using LC−ESI−UHR−QqTOF−MS, and a total of 82 and 15 compounds were identified, in negative and positive ionization modes, respectively. Organic acids were the main class of compounds identified in the phenolic-rich extract, followed by phenolic compounds, saccharides, peptides or amino acids and vitamins. Additionally, the extract revealed a significant antioxidant capacity (914.1 ± 51.6 and 2764.5 ± 142.8 µmol Trolox equivalents/g-dw, respectively, with ABTS and ORAC methodologies), which might be interesting for a wide variety of applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/pr10071339
dc.identifier.eid85137356504
dc.identifier.issn2227-9717
dc.identifier.urihttp://hdl.handle.net/10400.14/38311
dc.identifier.wos000832169800001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPhenolicspt_PT
dc.subjectAntioxidantspt_PT
dc.subjectSolid phase extractionpt_PT
dc.subjectLC-MSpt_PT
dc.subjectWaste/by-products valorizationpt_PT
dc.titleValorization of spent sugarcane fermentation broth as a source of phenolic compoundspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage15pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleProcessespt_PT
oaire.citation.volume10pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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