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Suitable simple and fast methods for selective isolation of phospholipids as a tool for their analysis

2018Journal article Restricted access
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dc.contributor.authorPimentel, Lígia
dc.contributor.authorFontes, Ana Luiza
dc.contributor.authorSalsinha, Sofia
dc.contributor.authorMachado, Manuela
dc.contributor.authorCorreia, Inês
dc.contributor.authorGomes, Ana Maria
dc.contributor.authorPintado, Manuela
dc.contributor.authorRodríguez-Alcalá, Luis Miguel
dc.date.accessioned2018-09-20T10:49:34Z
dc.date.available2018-09-20T10:49:34Z
dc.date.issued2018
dc.description.abstractLipids are gaining relevance over the last 20 years, as our knowledge about their role has changed from merely energy/structural molecules to compounds also involved in several biological processes. This led to the creation in 2003 of a new emerging research field: lipidomics. In particular the phospholipids have pharmacological/food applications, participate in cell signalling/homeostatic pathways while their analysis faces some challenges. Their fractionation/purification is, in fact, especially difficult, as they are amphiphilic compounds. Moreover, it usually involves SPE or TLC procedures requiring specific materials hampering their suitableness for routine analysis. Finally, they can interfere with the ionization of other molecules during mass spectrometry analysis. Thus, simple high‐throughput reliable methods to selectively isolate these compounds based on the difference between chemical characteristics of lipids would represent valuable tools for their study besides that of other compounds. The current review work aims to describe the state‐of‐the‐art related to the extraction of phospholipids using liquid‐liquid methods for their targeted isolation. The technological and biological importance of these compounds and ion suppression phenomena are also reviewed. Methods by precipitation with acetone or isolation using methanol seem to be suitable for selective isolation of phospholipids in both biological and food samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPimentel, L.L., Fontes, A.L., Salsinha, S., Machado, M., Correia, I., Gomes, A. M., Pintado, M. E., Rodríguez-Alcalá, L.M. (2018). Suitable simple and fast methods for selective isolation of phospholipids as a tool for their analysis. Electrophoresis, 39(15), 1835-1845pt_PT
dc.identifier.doi10.1002/elps.201700425pt_PT
dc.identifier.eid85044528051
dc.identifier.eissn1522-2683
dc.identifier.issn0173-0835
dc.identifier.pmid29518261
dc.identifier.urihttp://hdl.handle.net/10400.14/25717
dc.identifier.wos000440549300002
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley and Sonspt_PT
dc.relationNORTE-01-0145-FEDER-000030pt_PT
dc.relationPOCI-01-0247-FEDER-003466pt_PT
dc.relationImpact of microbial produced bioactive CLA and CLnA on acquired and genetic obesity and on gut microbiota in a murine model
dc.relationDevelopment of a new functional dairy product enriched in microbial bioactive conjugated fatty acids through an industrial-based approach
dc.subjectIon suppressionpt_PT
dc.subjectLLEpt_PT
dc.subjectPhospholipidspt_PT
dc.subjectSelective isolationpt_PT
dc.titleSuitable simple and fast methods for selective isolation of phospholipids as a tool for their analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImpact of microbial produced bioactive CLA and CLnA on acquired and genetic obesity and on gut microbiota in a murine model
oaire.awardTitleDevelopment of a new functional dairy product enriched in microbial bioactive conjugated fatty acids through an industrial-based approach
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-TEC%2F2125%2F2014/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.citation.endPage1845
oaire.citation.issue15
oaire.citation.startPage1835
oaire.citation.titleELECTROPHORESISpt_PT
oaire.citation.volume39
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNamePimentel
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person.familyNameGomes
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person.familyNameRodríguez-Alcalá
person.givenNameLígia
person.givenNameAna Luiza
person.givenNameAna Sofia
person.givenNameManuela
person.givenNameAna Maria
person.givenNameMaria Manuela
person.givenNameLuis Miguel
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project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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