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Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography–ion trap mass spectrometry

2014Journal article Restricted access
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dc.contributor.authorCarvalho, Luís Miguel
dc.contributor.authorCarvalho, Félix
dc.contributor.authorBastos, Maria de Lourdes
dc.contributor.authorBaptista, Paula
dc.contributor.authorMoreira, Nathalie
dc.contributor.authorMonforte, Ana Rita
dc.contributor.authorSilva, Ferreira António César
dc.contributor.authorPinho, Paula Guedes de
dc.date.accessioned2015-05-15T17:49:30Z
dc.date.available2015-05-15T17:49:30Z
dc.date.issued2014
dc.description.abstractMushrooms are known all over the world both due to the remarkable gastronomic value of some species and for severe intoxications mediated by other species that are frequently difficult to distinguish from the edible ones, by the common user. Therefore, it is important to develop strategies to discover molecules that can identify mushroom species. In the present work, two GC–MS methodologies were applied in the chemical characterization of 22 mushroom species (12 edible, 3 toxic and 7 potentially toxic) – a multi-target procedure to simultaneously determine amino acids (AA), fatty acids (FA) and sterols by previous derivatization procedure with MSTFA, and a Head Space-Solid Phase Microextraction method to determine volatiles. For both methods, two approaches to data analysis were used: (I) targeted analysis, to identify and quantify AA, FA sterols and volatiles; (II) untargeted analysis, including Principal Component Analysis and Partial Least Square Discriminant Analysis, in order to identify metabolites/metabolite pattern with potential species identification and/or differentiation. Multi-target experiment allowed the identification and quantification of twenty one primary metabolites (9 AA, 11 FA and 1 sterol). Furthermore, through untargeted data analysis, it was possible to identify a 5- carbon sugar alcohol structure molecule, which was tentatively identified as xylitol or adonitol, with potential to be a species-marker of the edible Suillus bovinus mushrooms. Volatile profiling studies resulted in the identification of the main volatiles in mushrooms. Untargeted analysis allowed the identification of 6 molecules that can be species- or genus-specific: one secondary metabolite specific to the edible species Lycoperdon perlatum, an ester of hexanoic acid, tentatively identified as allyl or vinyl caproate; and five other secondary metabolites, whose identification was not achieved, which were only detected in Lactarius aurantiacus specimens (edibility/toxicity unknown).por
dc.identifier.citationCARVALHO, Luís Miguel ...[et al.] - Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography–ion trap mass spectrometry. Talanta. ISSN 0039-9140. Vol. 118 (2014), p. 292–303por
dc.identifier.doi10.1016/j.talanta.2013.09.038
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/10400.14/17625
dc.identifier.wosWOS:000329553600038
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier
dc.relationStrategic Project - LA 6 - 2011-2012
dc.subjectEdible and toxic wild mushroomspor
dc.subjectHS-SPME GC-IT/MSpor
dc.subjectVolatilespor
dc.subjectSpecies discriminationpor
dc.subjectPrincipal Component Analysis (PCA)por
dc.subjectPartial Least Square Discriminantpor
dc.subjectAnalysis (PLS-DA)por
dc.titleNon-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography–ion trap mass spectrometrypor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 6 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FEQB%2FLA0006%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F63851%2F2009/PT
oaire.citation.endPage303
oaire.citation.startPage292
oaire.citation.titleTalanta
oaire.citation.volume118
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameCarvalho
person.familyNameBastos
person.familyNameBaptista
person.familyNameMoreira
person.familyNameMonforte
person.familyNameSilva Ferreira
person.familyNameGuedes de Pinho
person.givenNameFélix
person.givenNameMaria de Lourdes
person.givenNamePaula
person.givenNameNathalie
person.givenNameAna Rita
person.givenNameAntonio Cesar
person.givenNamePaula
person.identifier392870
person.identifier.ciencia-id491E-09B9-DBED
person.identifier.ciencia-idC31F-AAC1-24D5
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.ciencia-id471B-600E-ABC5
person.identifier.ciencia-id931A-48DA-23A8
person.identifier.ciencia-id2510-1418-EF48
person.identifier.orcid0000-0003-3858-3494
person.identifier.orcid0000-0001-9895-503X
person.identifier.orcid0000-0001-6331-3731
person.identifier.orcid0000-0002-4862-7510
person.identifier.orcid0000-0003-2627-2656
person.identifier.orcid0000-0002-1188-1021
person.identifier.orcid0000-0002-7395-5700
person.identifier.ridD-4914-2013
person.identifier.ridD-8588-2013
person.identifier.ridD-5498-2013
person.identifier.scopus-author-id7103070417
person.identifier.scopus-author-id25822248900
person.identifier.scopus-author-id14051688000
person.identifier.scopus-author-id55622494400
person.identifier.scopus-author-id6504448214
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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