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Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation

2021-05Journal article Open access
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dc.contributor.authorCoscueta, Ezequiel R.
dc.contributor.authorSousa, Ana Sofia
dc.contributor.authorReis, Celso A.
dc.contributor.authorPintado, Manuela
dc.date.accessioned2021-05-23T15:38:35Z
dc.date.available2021-05-23T15:38:35Z
dc.date.issued2021-05
dc.description.abstractPhenylethyl isothiocyanate (PEITC), a chemopreventive compound, is highly reactive due to its considerably electrophilic nature. Furthermore, it is hydrophobic and has low stability, bioavailability and bioaccessibility. This restricts its use in biomedical and nutraceutical or food applications. Thus, the encapsulation of this agent has the function of overcoming these limitations, promoting its solubility in water, and stabilizing it, preserving its bioactivity. So, polymeric microparticles were developed using chitosan-olive oil-PEITC systems. For this, an optimisation process (factors: olive oil: chitosan ratio and PEITC: chitosan ratio) was implemented through a 3-level factorial experimental design. The responses were: the particle size, zeta-potential, polydisperse index, and entrapment efficiency. The optimal formulation was further characterised by FTIR and biocompatibility in Caco-2 cells. Optimal conditions were olive oil: chitosan and PEITC: chitosan ratios of 1.46 and 0.25, respectively. These microparticles had a size of 629 nm, a zeta-potential of 32.3 mV, a polydispersity index of 0.329, and entrapment efficiency of 98.49%. We found that the inclusion process affected the optical behaviour of the PEITC, as well as the microparticles themselves and their interaction with the medium. Furthermore, the microparticles did not show cytotoxicity within the therapeutic values of PEITC. Thus, PEITC was microencapsulated with characteristics suitable for potential biomedical, nutraceutical and food applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1371/journal.pone.0248257pt_PT
dc.identifier.eid85105271765
dc.identifier.issn1932-6203
dc.identifier.pmcPMC8101728
dc.identifier.pmid33956792
dc.identifier.urihttp://hdl.handle.net/10400.14/33166
dc.identifier.wos000664611400009
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleChitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue5pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume16pt_PT
person.familyNameCoscueta
person.familyNameReis
person.familyNamePintado
person.givenNameEzequiel
person.givenNameCelso
person.givenNameMaria Manuela
person.identifier456608
person.identifier.ciencia-id6614-705D-EBA7
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-4496-2001
person.identifier.orcid0000-0002-0286-6639
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridJ-7033-2016
person.identifier.ridB-9969-2008
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id56986455000
person.identifier.scopus-author-id7103111131
person.identifier.scopus-author-id7004483898
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf1f94059-86c8-4386-875f-a864386d54b4
relation.isAuthorOfPublicationd6e69fb7-425e-4545-b604-4e323419f7d6
relation.isAuthorOfPublicationba387c7d-27c9-4016-895c-b35597e91ebc
relation.isAuthorOfPublication.latestForDiscoveryba387c7d-27c9-4016-895c-b35597e91ebc

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