Publication
Chlorella vulgaris extracts as modulators of the health status and the inflammatory response of gilthead seabream juveniles (Sparus aurata)
| dc.contributor.author | Reis, Bruno | |
| dc.contributor.author | Ramos-Pinto, Lourenço | |
| dc.contributor.author | Cunha, Sara A. | |
| dc.contributor.author | Pintado, Manuela | |
| dc.contributor.author | Silva, Joana Laranjeira da | |
| dc.contributor.author | Dias, Jorge | |
| dc.contributor.author | Conceição, Luís | |
| dc.contributor.author | Matos, Elisabete | |
| dc.contributor.author | Costas, Benjamín | |
| dc.date.accessioned | 2022-07-22T12:32:12Z | |
| dc.date.available | 2022-07-22T12:32:12Z | |
| dc.date.issued | 2022-07 | |
| dc.description.abstract | This study aimed to evaluate the effects of short-term supplementation, with 2% Chlorella vulgaris (C. vulgaris) biomass and two 0.1% C. vulgaris extracts, on the health status (experiment one) and on the inflammatory response (experiment two) of gilthead seabream (Sparus aurata). The trial comprised four isoproteic (50% crude protein) and isolipidic (17% crude fat) diets. A fishmeal-based (FM), practical diet was used as a control (CTR), whereas three experimental diets based on CTR were further supplemented with a 2% inclusion of C. vulgaris biomass (Diet D1); 0.1% inclusion of C. vulgaris peptide-enriched extract (Diet D2) and finally a 0.1% inclusion of C. vulgaris insoluble fraction (Diet D3). Diets were randomly assigned to quadruplicate groups of 97 fish/tank (IBW: 33.4 ± 4.1 g), fed to satiation three times a day in a recirculation seawater system. In experiment one, seabream juveniles were fed for 2 weeks and sampled for tissues at 1 week and at the end of the feeding period. Afterwards, randomly selected fish from each group were subjected to an inflammatory insult (experiment two) by intraperitoneal injection of inactivated gram-negative bacteria, following 24 and 48 h fish were sampled for tissues. Blood was withdrawn for haematological pro-cedures, whereas plasma and gut tissue were sampled for immune and oxidative stress parameters. The anterior gut was also collected for gene expression measurements. After 1 and 2 weeks of feed-ing, fish fed D2 showed higher circulating neutrophils than seabream fed CTR. In contrast, dietary treatments induced mild effects on the innate immune and antioxidant functions of gilthead sea-bream juveniles fed for 2 weeks. In the inflammatory response following the inflammatory insult, mild effects could be attributed to C. vulgaris supplementation either in biomass form or extract. However, the C. vulgaris soluble peptide-enriched extract seems to confer a protective, anti-stress effect in the gut at the molecular level, which should be further explored in future studies. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.3390/md20070407 | pt_PT |
| dc.identifier.eid | 85132982855 | |
| dc.identifier.issn | 1660-3397 | |
| dc.identifier.pmc | PMC9323325 | |
| dc.identifier.pmid | 35877700 | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/38357 | |
| dc.identifier.wos | 000833321200001 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Fish robustness | pt_PT |
| dc.subject | Functional feeds | pt_PT |
| dc.subject | Innate immunity | pt_PT |
| dc.subject | Protein hydrolysate | pt_PT |
| dc.title | Chlorella vulgaris extracts as modulators of the health status and the inflammatory response of gilthead seabream juveniles (Sparus aurata) | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 7 | pt_PT |
| oaire.citation.title | Marine Drugs | pt_PT |
| oaire.citation.volume | 20 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |