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Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS

2014Journal article Open access
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dc.contributor.authorBranquinho, Raquel
dc.contributor.authorSousa, Clara
dc.contributor.authorLopes, João
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorPeixe, Luísa V.
dc.contributor.authorOsório, Hugo
dc.date.accessioned2015-05-11T17:03:08Z
dc.date.available2015-05-11T17:03:08Z
dc.date.issued2014
dc.description.abstractMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. Bacillus pumillus and Bacillus safensis, are species of biotechnological and pharmaceutical significance, difficult to differentiate by conventional methodologies. In this study, using a well-characterized collection of B. pumillus and B. safensis isolates, we demonstrated the suitability of MALDI-TOF-MS combined with chemometrics to accurately and rapidly identify them. Moreover, characteristic species-specific ion masses were tentatively assigned, using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and primary literature. Delineation of B. pumilus (ions at m/z 5271 and 6122) and B. safensis (ions at m/z 5288, 5568 and 6413) species were supported by a congruent characteristic protein pattern. Moreover, using a chemometric approach, the score plot created by partial least square discriminant analysis (PLSDA) of mass spectra demonstrated the presence of two individualized clusters, each one enclosing isolates belonging to a species-specific spectral group. The generated pool of species-specific proteins comprised mostly ribosomal and SASPs proteins. Therefore, in B. pumilus the specific ion at m/z 5271 was associated with a small acid-soluble spore protein (SASP O) or with 50S protein L35, whereas in B. safensis specific ions at m/z 5288 and 5568 were associated with SASP J and P, respectively, and an ion at m/z 6413 with 50S protein L32. Thus, the resulting unique protein profile combined with chemometric analysis, proved to be valuable tools for B. pumilus and B. safensis discrimination, allowing their reliable, reproducible and rapid identification.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationBRANQUINHO, Raquel ...[et al.] - Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS. PLOS ONE. ISSN 1932-6203. Vol. 9 n.º 10, e110127 (2014), , p. 1-10por
dc.identifier.doi10.1371/journal.pone.0110127
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.14/17548
dc.identifier.wosWOS:000343662500099
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPublic Library of Science
dc.relationGENOTYPIC AND HIGH-TROUGHPUT METHODOLOGIES FOR TRACING THE EMERGING PATHOGEN ESCHERICHIA ALBERTII
dc.titleDifferentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MSpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleGENOTYPIC AND HIGH-TROUGHPUT METHODOLOGIES FOR TRACING THE EMERGING PATHOGEN ESCHERICHIA ALBERTII
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F61410%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F70548%2F2010/PT
oaire.citation.issue10
oaire.citation.titlePLoS ONE
oaire.citation.volume9
oaire.fundingStreamSFRH
person.familyNameBranquinho
person.familyNameSantana Sousa
person.familyNameLopes
person.familyNamePintado
person.familyNamePeixe
person.familyNameOsorio
person.givenNameFabiana
person.givenNameClara Cecília
person.givenNameJoao
person.givenNameMaria Manuela
person.givenNameLuísa
person.givenNameHugo
person.identifier126281
person.identifier456608
person.identifier.ciencia-id3510-BBAC-9407
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person.identifier.orcid0000-0002-1629-9292
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person.identifier.orcid0000-0002-1657-344X
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person.identifier.orcid0000-0002-6362-8255
person.identifier.ridC-6789-2019
person.identifier.ridH-1733-2011
person.identifier.ridF-5696-2013
person.identifier.ridH-7347-2016
person.identifier.scopus-author-id55699847900
person.identifier.scopus-author-id7006164633
person.identifier.scopus-author-id7202733600
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id6603892851
person.identifier.scopus-author-id6601979151
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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