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Diverse geno- and phenotypes of persistent listeria monocytogenes isolates from fermented meat sausage production facilities in Portugal

2011Journal article Open access
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dc.contributor.authorFerreira, V.
dc.contributor.authorBarbosa, J.
dc.contributor.authorStasiewicz, M.
dc.contributor.authorVongkamjan, K.
dc.contributor.authorSwitt, A. Moreno
dc.contributor.authorHogg, T.
dc.contributor.authorGibbs, P.
dc.contributor.authorTeixeira, P.
dc.contributor.authorWiedmann, M.
dc.date.accessioned2012-02-27T15:47:20Z
dc.date.available2012-02-27T15:47:20Z
dc.date.issued2011
dc.description.abstractThe persistence of Listeria monocytogenes in food-associated environments represents a key factor in transmission of this pathogen. To identify persistent and transient strains associated with production of fermented meat sausages in northern Portugal, 1,723 L. monocytogenes isolates from raw material and finished products from 11 processors were initially characterized by random amplification of polymorphic DNA (RAPD), PCR-based molecular serotyping, and epidemic clone characterization, as well as cadmium, arsenic, and tetracycline resistance typing. Pulsed-field gel electrophoresis (PFGE) typing of 240 representative isolates provided evidence for persistence of L. monocytogenes for periods of time ranging from 10 to 32 months for all seven processors for which isolates from different production dates were available. Among 50 L. monocytogenes isolates that included one representative for each PFGE pattern obtained from a given sample, 12 isolates showed reduced invasion efficiency in Caco-2 cells, including 8 isolates with premature stop codons in inlA. Among 41 isolates representing sporadic and persistent PFGE types, 22 isolates represented lysogens. Neither strains with reduced invasion nor lysogens were overrepresented among persistent isolates. While the susceptibility of isolates to lysogenic phages also did not correlate with persistence, it appeared to be associated with molecular serotype. Our data show the following. (i) RAPD may not be suitable for analysis of large sets of L. monocytogenes isolates. (ii) While a large diversity of L. monocytogenes subtypes is found in Portuguese fermented meat sausages, persistence of L. monocytogenes in this food chain is common. (iii) Persistent L. monocytogenes strains are diverse and do not appear to be characterized by unique genetic or phenotypic characteristics.por
dc.description.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.citationFERREIRA, V... [et al.] - Diverse geno- and phenotypes of persistent listeria monocytogenes isolates from fermented meat sausage production facilities in Portugal. Applied and Environmental Microbiology. ISSN 0099-2240. Vol. 77, n.º 8 (2011), p. 2701-2715por
dc.identifier.doi10.1128/AEM.02553-10
dc.identifier.eissn1098-5336
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10400.14/7957
dc.identifier.wosWOS:000289459300018
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Society for Microbiologypor
dc.relation2005-51110-03278
dc.relation2006-34459-16952
dc.relation2008-34459-19043
dc.relationNYC-143445
dc.relationTradition, Quality & Safety- traditional fermented meat products.
dc.titleDiverse geno- and phenotypes of persistent listeria monocytogenes isolates from fermented meat sausage production facilities in Portugalpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTradition, Quality & Safety- traditional fermented meat products.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FAGG%2F39587%2F2001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-ALI%2F64662%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F24240%2F2005/PT
oaire.citation.endPage2715
oaire.citation.issue8
oaire.citation.startPage2701
oaire.citation.titleApplied and Environmental Microbiology
oaire.citation.volume77
oaire.fundingStreamPOCI
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameBorges Ferreira
person.familyNameBarbosa
person.familyNameStasiewicz
person.familyNameMoreno Switt
person.familyNameHogg
person.familyNameGibbs
person.familyNameTeixeira
person.familyNameWiedmann
person.givenNameVânia
person.givenNameJoana
person.givenNameMatthew
person.givenNameAndrea
person.givenNameTim
person.givenNamePaul
person.givenNamePaula
person.givenNameMartin
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person.identifier.ridK-1343-2014
person.identifier.ridA-9083-2012
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person.identifier.ridJ-8678-2014
person.identifier.ridA-9683-2008
person.identifier.scopus-author-id8317338900
person.identifier.scopus-author-id13608752600
person.identifier.scopus-author-id23502454200
person.identifier.scopus-author-id7005603860
person.identifier.scopus-author-id7005895206
person.identifier.scopus-author-id7006030315
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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