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Kinetic determination of L (-)malic acid in wines using sequential injection analysis

dc.contributor.authorSegundo, Marcela A.
dc.contributor.authorRangel, António O. S. S.
dc.date.accessioned2011-10-22T12:41:44Z
dc.date.available2011-10-22T12:41:44Z
dc.date.issued2003
dc.description.abstractIn the present work a sequential injection system for the enzymatic determination of Image (−)malic acid in wines is described. The determination was based on the detection of NADH, formed by oxidation of Image -malate to oxaloacetate, catalysed by Image -malate dehydrogenase. A kinetic method was implemented by stopping the flow when a solution plug containing sample, NAD+ and enzyme reached the detector. The rate of reaction was measured by monitoring absorbance changes at 340 nm during a fixed period of time. A linear calibration curve (rate of reaction versus concentration) was obtained for concentrations of Image (−)malic acid between 0.01 and 0.15 g l−1. The determination rate was about 22 h−1 and samples were diluted 25 or 50 times before introduction to the system. Results obtained from 16 wine samples were comparable to those obtained by the reference method.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationSEGUNDO, Marcela A. ; RANGEL, António O.S.S. - Kinetic determination of Image (−)malic acid in wines using sequential injection analysis. Analytica Chimica Acta. ISSN 0003-2670. Vol. 499, n.º1-2 (2003) p. 99-106por
dc.identifier.urihttp://hdl.handle.net/10400.14/6820
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectSequential injection analysispor
dc.subjectMalic acidpor
dc.subjectKinetic enzymatic determinationpor
dc.subjectWinespor
dc.titleKinetic determination of L (-)malic acid in wines using sequential injection analysispor
dc.typejournal article
dspace.entity.typePublication
person.familyNameSegundo
person.familyNameSantos Silva Rangel
person.givenNameMarcela
person.givenNameAntónio Osmaro
person.identifier.ciencia-id0A14-B7A6-E805
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0003-2938-0214
person.identifier.orcid0000-0002-6486-8947
person.identifier.ridA-1009-2010
person.identifier.scopus-author-id6602384941
person.identifier.scopus-author-id7004827387
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication84c05a53-23fa-4f4b-b007-d1a5fc748d5a
relation.isAuthorOfPublication467be022-fd9c-4549-aa48-48ebcc1098eb
relation.isAuthorOfPublication.latestForDiscovery467be022-fd9c-4549-aa48-48ebcc1098eb

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