Publication
Kinetic determination of L (-)malic acid in wines using sequential injection analysis
dc.contributor.author | Segundo, Marcela A. | |
dc.contributor.author | Rangel, António O. S. S. | |
dc.date.accessioned | 2011-10-22T12:41:44Z | |
dc.date.available | 2011-10-22T12:41:44Z | |
dc.date.issued | 2003 | |
dc.description.abstract | In the present work a sequential injection system for the enzymatic determination of Image (−)malic acid in wines is described. The determination was based on the detection of NADH, formed by oxidation of Image -malate to oxaloacetate, catalysed by Image -malate dehydrogenase. A kinetic method was implemented by stopping the flow when a solution plug containing sample, NAD+ and enzyme reached the detector. The rate of reaction was measured by monitoring absorbance changes at 340 nm during a fixed period of time. A linear calibration curve (rate of reaction versus concentration) was obtained for concentrations of Image (−)malic acid between 0.01 and 0.15 g l−1. The determination rate was about 22 h−1 and samples were diluted 25 or 50 times before introduction to the system. Results obtained from 16 wine samples were comparable to those obtained by the reference method. | por |
dc.description.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.citation | SEGUNDO, Marcela A. ; RANGEL, António O.S.S. - Kinetic determination of Image (−)malic acid in wines using sequential injection analysis. Analytica Chimica Acta. ISSN 0003-2670. Vol. 499, n.º1-2 (2003) p. 99-106 | por |
dc.identifier.uri | http://hdl.handle.net/10400.14/6820 | |
dc.language.iso | eng | por |
dc.peerreviewed | yes | por |
dc.publisher | Elsevier | por |
dc.subject | Sequential injection analysis | por |
dc.subject | Malic acid | por |
dc.subject | Kinetic enzymatic determination | por |
dc.subject | Wines | por |
dc.title | Kinetic determination of L (-)malic acid in wines using sequential injection analysis | por |
dc.type | journal article | |
dspace.entity.type | Publication | |
person.familyName | Segundo | |
person.familyName | Santos Silva Rangel | |
person.givenName | Marcela | |
person.givenName | António Osmaro | |
person.identifier.ciencia-id | 0A14-B7A6-E805 | |
person.identifier.ciencia-id | D61D-7CFB-178A | |
person.identifier.orcid | 0000-0003-2938-0214 | |
person.identifier.orcid | 0000-0002-6486-8947 | |
person.identifier.rid | A-1009-2010 | |
person.identifier.scopus-author-id | 6602384941 | |
person.identifier.scopus-author-id | 7004827387 | |
rcaap.rights | restrictedAccess | por |
rcaap.type | article | por |
relation.isAuthorOfPublication | 84c05a53-23fa-4f4b-b007-d1a5fc748d5a | |
relation.isAuthorOfPublication | 467be022-fd9c-4549-aa48-48ebcc1098eb | |
relation.isAuthorOfPublication.latestForDiscovery | 467be022-fd9c-4549-aa48-48ebcc1098eb |