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Influence of PLLA/PCL/HA scaffold fiber orientation on mechanical properties and osteoblast behavior

2019Journal article Open access
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dc.contributor.authorSiqueira, Lilian de
dc.contributor.authorRibeiro, Nilza
dc.contributor.authorParedes, Maria B. A.
dc.contributor.authorGrenho, Liliana
dc.contributor.authorCunha-Reis, Cassilda
dc.contributor.authorTrichês, Eliandra S.
dc.contributor.authorFernandes, Maria H.
dc.contributor.authorSousa, Susana R.
dc.contributor.authorMonteiro, Fernando J.
dc.date.accessioned2019-12-06T11:51:53Z
dc.date.available2019-12-06T11:51:53Z
dc.date.issued2019
dc.description.abstractScaffolds based on aligned and non-aligned poly (L-lactic acid) (PLLA)/polycaprolactone (PCL) fibers obtained by electrospinning, associated to electrosprayed hydroxyapatite (HA) for tissue engineering applications were developed and their performance was compared in terms of their morphology and biological and mechanical behaviors. The morphological results assessed by scanning electron microscopy showed a mesh of PLLA/PCL fibers (random and perfectly aligned) associated with aggregates of nanophased HA. Fourier transform infrared spectrometry confirmed the homogeneity in the blends and the presence of nanoHA in the scaffold. As a result of fiber alignment a 15-fold increase in Young’s Modulus and an 8-fold increase in tensile strength were observed when compared to non-aligned fibers. In PLLA/PCL/HA scaffolds, the introduction of nanoHA caused a remarkable improvement of the mechanical strength of this material acting as a reinforcement, enhancing the response of these constructs to tensile stress. In vitro testing was evaluated using osteoblast (MC3T3-E1) cells. The results showed that both fibrous scaffolds were able to support osteoblast cell adhesion and proliferation and that fiber alignment induced increased cellular metabolic activity. In addition, the adhesion and proliferation of Staphylococcus aureus were evaluated and a lower number of colony forming units (CFUs) was obtained in the scaffolds with aligned fibers.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSiqueira, L., Ribeiro, N., Paredes, M., Grenho, L., Cunha-Reis, C., Trichês, E. S., ..., Monteiro, F. J. (2019). Influence of PLLA/PCL/HA Scaffold Fiber Orientation on Mechanical Properties and Osteoblast Behavior. Materials, 12(23), 3879pt_PT
dc.identifier.doi10.3390/ma12233879pt_PT
dc.identifier.eid85075898783
dc.identifier.issn1996-1944
dc.identifier.pmcPMC6926818
dc.identifier.pmid31771297
dc.identifier.urihttp://hdl.handle.net/10400.14/28957
dc.identifier.wos000510178700075
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationInstitute for Research and Innovation in Health Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBone-like biomaterialpt_PT
dc.subjectPLLA/PCL/HA scaffoldspt_PT
dc.subjectNanohydroxyapatitept_PT
dc.subjectElectrospinning/electrospraying techniquept_PT
dc.subjectRandom and aligned fiberspt_PT
dc.subjectMechanical propertiespt_PT
dc.subjectOsteoblastspt_PT
dc.titleInfluence of PLLA/PCL/HA scaffold fiber orientation on mechanical properties and osteoblast behaviorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute for Research and Innovation in Health Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F04293%2F2019/PT
oaire.citation.issue23
oaire.citation.titleMaterialspt_PT
oaire.citation.volume12
oaire.fundingStream6817 - DCRRNI ID
person.familyNamede Siqueira
person.familyNameArteaga Paredes
person.familyNameCunha Reis
person.familyNameMonteiro
person.givenNameLilian
person.givenNameMaria Belen
person.givenNameCassilda
person.givenNameFernando
person.identifier.ciencia-idE718-0A09-A959
person.identifier.ciencia-id5B1E-8842-AD62
person.identifier.orcid0000-0003-1334-3312
person.identifier.orcid0000-0001-5965-8630
person.identifier.orcid0000-0001-6886-3900
person.identifier.orcid0000-0002-1361-4605
person.identifier.ridU-3671-2018
person.identifier.scopus-author-id20336729000
person.identifier.scopus-author-id7006030702
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication99d601d6-7c3d-4924-bcc8-0b6ebbf1d694
relation.isAuthorOfPublication8e562eb9-a71e-4a86-bdbd-cf556f3cfe05
relation.isAuthorOfPublication3000922a-5ab0-4d36-8fd3-e88bfca427f1
relation.isAuthorOfPublication7412d117-06a8-4dc4-b54a-f072d3002a25
relation.isAuthorOfPublication.latestForDiscovery8e562eb9-a71e-4a86-bdbd-cf556f3cfe05
relation.isProjectOfPublication562a87c2-d2a0-41df-8bd7-713df31a95ea
relation.isProjectOfPublication.latestForDiscovery562a87c2-d2a0-41df-8bd7-713df31a95ea

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