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Vaginal prevention of Candida albicans: synergistic effect of lactobacilli and mannan oligosaccharides (MOS)

2024-12-01Journal article Open access
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dc.contributor.authorFaustino, Margarida
dc.contributor.authorPereira, Joana Odila
dc.contributor.authorPereira, Ana Margarida
dc.contributor.authorOliveira, Ana Sofia
dc.contributor.authorFerreira, Carlos M. H.
dc.contributor.authorPereira, Carla F.
dc.contributor.authorDurão, Joana
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorCarvalho, Ana P.
dc.date.accessioned2024-01-17T16:11:45Z
dc.date.available2024-01-17T16:11:45Z
dc.date.issued2024-12-01
dc.description.abstractVulvovaginal candidiasis (VVC) affects approximately 30-50% of women at least once during their lifetime, causing uncomfortable symptoms and limitations in their daily quality of life. Antifungal therapy is not very effective, does not prevent recurrencies and usually causes side effects. Therefore, alternative therapies are urgently needed. The goal of this work was to investigate the potential benefits of using mannan oligosaccharides (MOS) extracts together with a Lactobacillus sp. pool, composed by the most significant species present in the vaginal environment, to prevent infections by Candida albicans. Microbial growth of isolated strains of the main vaginal lactobacilli and Candida strains was assessed in the presence of MOS, to screen their impact upon growth. A pool of the lactobacilli was then tested against C. albicans in competition and prophylaxis studies; bacterial and yeast cell numbers were quantified in specific time points, and the above-mentioned studies were assessed in simulated vaginal fluid (SVF). Finally, adhesion to vaginal epithelial cells (HeLa) was also evaluated, once again resorting to simultaneous exposure (competition) or prophylaxis assays, aiming to measure the effect of MOS presence in pathogen adherence. Results demonstrated that MOS extracts have potential to prevent vaginal candidiasis in synergy with vaginal lactobacilli, with improved results than those obtained when using lactobacilli alone. KEY POINTS: Potential benefits of MOS extracts with vaginal lactobacilli to prevent C. albicans infections. MOS impacts on growth of vaginal lactobacilli pool and C. albicans in SVF. MOS extracts in synergy with L. crispatus inhibit C. albicans adhesion in HeLa cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00253-023-12909-2pt_PT
dc.identifier.eid85182000825
dc.identifier.issn0175-7598
dc.identifier.pmcPMC10776728
dc.identifier.pmid38194142
dc.identifier.urihttp://hdl.handle.net/10400.14/43660
dc.identifier.wos001284745400011
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCompetition assayspt_PT
dc.subjectHeLa cellspt_PT
dc.subjectLactobacillus crispatuspt_PT
dc.subjectMannan oligosaccharidespt_PT
dc.subjectProphylaxis assayspt_PT
dc.subjectVaginal therapeuticspt_PT
dc.titleVaginal prevention of Candida albicans: synergistic effect of lactobacilli and mannan oligosaccharides (MOS)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.titleApplied Microbiology and Biotechnologypt_PT
oaire.citation.volume108pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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