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Rapid spectrophotometric determination of nitrates and nitrites in marine aqueous culture media

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dc.contributor.authorCarvalho, A. P.
dc.contributor.authorMeireles, L. A.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-21T13:18:40Z
dc.date.available2011-10-21T13:18:40Z
dc.date.issued1998
dc.description.abstractThe spectrophotometric determination of nitrate in sea water broths for cultivation of, say, microalgae is complicated by the frequent presence of nitrite. Two methods - sulphamic/perchloric acid method (also known as Cawse method) and sulphamic acid method - both claimed to be able to eliminate nitrite interference, were tested using a set of standards, but statistical treatment of the results proved their limitations in nitrate quantification. An improved method, based on former published methods for quantification of nitrite and coupled determination of nitrate and nitrite, was designed and tested. This improved method was compared with the reference method (based on use of a cadmium column) using several standards and biological samples of two culture media for microalgae, in different phases of their growth curve. The results thus obtained have demonstrated that there is no statistically significant difference between them at the 5% level. The precision of the method was tested by repeating determinations with three sets of standard mixtures containing nitrate and nitrite. The method proposed has advantages over conventional methods in reduced time of analysis, as well as high precision and accuracy, so it may be a good alternative for determination of nitrite and nitrate in marine aqueous media.por
dc.identifier.citationCARVALHO, A. P.; MEIRELES, L. A.; MALCATA, F.X. - Rapid spectrophotometric determination of nitrates and nitrites in marine aqueous culture media. Analusis. ISSN 0365-4877. Vol. 26 (1998), p. 347-351por
dc.identifier.doi10.1051/analusis:1998183
dc.identifier.urihttp://hdl.handle.net/10400.14/6669
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherEDP Sciences, Wiley-VCHpor
dc.subjectEnriched sea water microalgae brothpor
dc.subjectNitrate quantificationpor
dc.subjectNitrite quantificationpor
dc.subjectSpectrophotometrypor
dc.titleRapid spectrophotometric determination of nitrates and nitrites in marine aqueous culture mediapor
dc.typejournal article
dspace.entity.typePublication
person.familyNameCarvalho
person.familyNameMalcata
person.givenNameAna
person.givenNameFrancisco
person.identifierK-5479-2013
person.identifier.ciencia-id6F1E-301F-71BA
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0001-5967-4200
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridK-5479-2013
person.identifier.scopus-author-id36855267200
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication61e93e5c-d206-455d-84cb-d5cbc048ee5b
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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