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Advisor(s)
Abstract(s)
Butterfat was chemically modified via combined hydrolysis and interesterification, catalyzed by a commercial lipase immobilized onto a bundle of hydrophobic hollow fibers.
The main goal of this research effort was to engineer butterfat with improved nutritional properties by taking advantage of the
sn-1,3 specificity and fatty acid specificity of a lipase in hydrolysis
and ester interchange reactions, and concomitantly decrease its level of long-chain saturated fatty acid residues (viz., lauric, myristic, and palmitic acids) and change its melting properties. All reactions were carried out at 40°C in a solvent free system under controlled water activity, and their extent was monitored via chromatographic assays for free fatty acids, esterified fatty acid moieties, and triacylglycerols; the thermal behavior of the modified butterfat was also assessed via calorimetry.
Lipase-modified butterfat possesses a wider melting temperature range than regular butterfat. The total saturated triacylglycerols decreased by 2.2%, whereas triacylglycerols with 28–46 acyl carbons (which contained two or three lauric, myristic, or palmitic acid moieties) decreased by 13%. The total monoene triacylglycerols increased by 5.4%, whereas polyene triacylglycerols decreased by 2.9%. The triacylglycerols of interesterified
butterfat had ca. 10.9% less lauric, 10.7% less myristic, and 13.6% less palmitic acid residues than those of the original butterfat.
Description
Keywords
Fats Hollow-fiber bioreactor Immobilized lipase Interesterification Melting point Mucor javanicus
Pedagogical Context
Citation
BALCÃO, Victor M....[et al] - Modification of butterfat by selective hydrolysis and interesterification by lipase: Process and product characterization . Journal of the American Oil Chemists' Society. ISSN 0003-021X. Vol. 75, n. 10 (1998), p. 1347-1358
Publisher
Springer