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Novel whey-derived peptides with inhibitory effect against angiotensin-converting enzyme: in vitro effect and stability to gastrointestinal enzymes

2011Journal article Restricted access
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dc.contributor.authorTavares, Tânia
dc.contributor.authorDel Mar Contreras, Maria
dc.contributor.authorAmorim, Manuela
dc.contributor.authorPintado, Manuela
dc.contributor.authorRecio, Esidra
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2012-02-27T16:11:14Z
dc.date.available2012-02-27T16:11:14Z
dc.date.issued2011
dc.description.abstractWhey protein concentrate (WPC) was subjected to enzymatic hydrolysis by proteases from the flowers of Cynara cardunculus, and the resulting angiotensin-converting enzyme (ACE)-inhibitory effect was monitored. The whole WPC hydrolysate exhibited an IC50 value of 52.9±2.9ºg/mL, whereas the associated peptide fraction with molecular weight below 3 kDa scored 23.6±1.1ºg/mL. The latter fraction was submitted to RP-HPLC, and 6 fractions were resolved that exhibited ACE-inhibitory effects. Among the various peptides found, a total of 14 were identified via sequencing with an ion-trap mass spectrometer. Eleven of these peptides were synthesized de novo – to validate their ACE-inhibitory effect, and also to ascertain their stability when exposed to simulated gastrointestinal digestion. Among them, three novel, highly potent peptides were found, corresponding to B-lactalbumin f(16–26) – with the sequence KGYGGVSLPEW, B-lactalbumin f(97–104) with DKVGINYW, and B-lactoglobulin f(33–42) with DAQSAPLRVY; their IC50 values were as low as 0.80±0.1, 25.2±1.0 and 13.0±1.0g/mL, respectively. None of them remained stable in the presence of gastrointestinal enzymes: they were partially, or even totally hydrolyzed to smaller peptides – yet the observed ACE-inhibitory effects were not severely affected for two of those peptides.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationTAVARES, Tânia... [et al.] - Novel whey-derived peptides with inhibitory effect against angiotensin-converting enzyme: in vitro effect and stability to gastrointestinal enzymes. Peptides. ISSN 0196-9781. Vol. 32, n.º 5 (2011), p. 1013-1019por
dc.identifier.eissn1873-5169
dc.identifier.issn0196-9781
dc.identifier.urihttp://hdl.handle.net/10400.14/7963
dc.identifier.wosWOS:000291286200024
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationAGL2007-65035
dc.relationAGL2008-01713
dc.relation2007PT0033
dc.subjectACE-inhibitory peptidepor
dc.subjectGastrointestinal digestionpor
dc.subjectHydrolyzed wheypor
dc.subjectAntihypertensive activitypor
dc.titleNovel whey-derived peptides with inhibitory effect against angiotensin-converting enzyme: in vitro effect and stability to gastrointestinal enzymespor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F31604%2F2006/PT
oaire.citation.endPage1019
oaire.citation.issue5
oaire.citation.startPage1013
oaire.citation.titlePeptides
oaire.citation.volume32
oaire.fundingStreamSFRH
person.familyNameCONTRERAS
person.familyNameAmorim
person.familyNamePintado
person.familyNameRecio
person.familyNameMalcata
person.givenNameMARIA DEL MAR
person.givenNameMaria Manuela
person.givenNameMaria Manuela
person.givenNameIsidra
person.givenNameFrancisco
person.identifier362150
person.identifier456608
person.identifier.ciencia-id791D-E9BA-D432
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0002-3407-0088
person.identifier.orcid0000-0002-1485-0423
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-2973-5329
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridK-9122-2014
person.identifier.ridL-4327-2014
person.identifier.ridF-5696-2013
person.identifier.ridH-4057-2012
person.identifier.scopus-author-id26653952000
person.identifier.scopus-author-id55293251500
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id7102542478
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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