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Enzymatically cross-linked silk fibroin-based hierarchical scaffolds for osteochondral regeneration

2019Journal article Restricted access
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dc.contributor.authorRibeiro, Viviana P.
dc.contributor.authorPina, Sandra
dc.contributor.authorCosta, João B.
dc.contributor.authorCengiz, Ibrahim Fatih
dc.contributor.authorGarcía-Fernández, Luis
dc.contributor.authorFernández-Gutiérrez, Maria del Mar
dc.contributor.authorPaiva, Olga C.
dc.contributor.authorOliveira, Ana L.
dc.contributor.authorSan-Román, Julio
dc.contributor.authorOliveira, Joaquim M.
dc.contributor.authorReis, Rui L.
dc.date.accessioned2019-07-11T10:45:55Z
dc.date.available2019-07-11T10:45:55Z
dc.date.issued2019
dc.description.abstractOsteochondral (OC) regeneration faces several limitations in orthopedic surgery, owing to the complexity of the OC tissue that simultaneously entails the restoration of articular cartilage and subchondral bone diseases. In this study, novel biofunctional hierarchical scaffolds composed of a horseradish peroxidase (HRP)-cross-linked silk fibroin (SF) cartilage-like layer (HRP-SF layer) fully integrated into a HRP-SF/ZnSr-doped β-tricalcium phosphate (β-TCP) subchondral bone-like layer (HRP-SF/dTCP layer) were proposed as a promising strategy for OC tissue regeneration. For comparative purposes, a similar bilayered structure produced with no ion incorporation (HRP-SF/TCP layer) was used. A homogeneous porosity distribution was achieved throughout the scaffolds, as shown by micro-computed tomography analysis. The ion-doped bilayered scaffolds presented a wet compressive modulus (226.56 ± 60.34 kPa) and dynamic mechanical properties (ranging from 403.56 ± 111.62 to 593.56 ± 206.90 kPa) superior to that of the control bilayered scaffolds (189.18 ± 90.80 kPa and ranging from 262.72 ± 59.92 to 347.68 ± 93.37 kPa, respectively). Apatite crystal formation, after immersion in simulated body fluid (SBF), was observed in the subchondral bone-like layers for the scaffolds incorporating TCP powders. Human osteoblasts (hOBs) and human articular chondrocytes (hACs) were co-cultured onto the bilayered structures and monocultured in the respective cartilage and subchondral bone half of the partitioned scaffolds. Both cell types showed good adhesion and proliferation in the scaffold compartments, as well as adequate integration of the interface regions. Osteoblasts produced a mineralized extracellular matrix (ECM) in the subchondral bone-like layers, and chondrocytes showed GAG deposition. The gene expression profile was different in the distinct zones of the bilayered constructs, and the intermediate regions showed pre-hypertrophic chondrocyte gene expression, especially on the BdTCP constructs. Immunofluorescence analysis supported these observations. This study showed that the proposed bilayered scaffolds allowed a specific stimulation of the chondrogenic and osteogenic cells in the co-culture system together with the formation of an osteochondral-like tissue interface. Hence, the structural adaptability, suitable mechanical properties, and biological performance of the hierarchical scaffolds make these constructs a desired strategy for OC defect regeneration.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRibeiro, V. P., Pina, S., Costa, J. B., Cengiz, I. F., García-Fernández, L., Fernández-Gutiérrez, M. del M., … Reis, R. L. (2019). Enzymatically Cross-Linked Silk Fibroin-Based Hierarchical Scaffolds for Osteochondral Regeneration. ACS Applied Materials & Interfaces, 11(4), 3781–3799. https://doi.org/10.1021/acsami.8b21259pt_PT
dc.identifier.doi10.1021/acsami.8b21259pt_PT
dc.identifier.eid85060315155
dc.identifier.issn1944-8244
dc.identifier.pmid30609898
dc.identifier.urihttp://hdl.handle.net/10400.14/27926
dc.identifier.wos000457816900014
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationPD/S9/2013
dc.relationTissue engineering approaches for regeneration of human articulation
dc.relationAdvanced tissue engineering strategies for biofabrication of complx tissues
dc.relationBioengineered Silk Fibroin-Based Contructs with Potential for Osteochondral Tissue Regeneration
dc.relationIF/00423/2012
dc.relationIF/01285/2015
dc.relationIF/00411/2013
dc.subjectHorseradish peroxidase-mediated cross-linkingpt_PT
dc.subjectSilk fibroinpt_PT
dc.subjectIon-doped β-tricalcium phosphatept_PT
dc.subjectBilayered scaffoldpt_PT
dc.subjectHierarchical structurept_PT
dc.subjectOsteochondral tissue engineeringpt_PT
dc.titleEnzymatically cross-linked silk fibroin-based hierarchical scaffolds for osteochondral regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTissue engineering approaches for regeneration of human articulation
oaire.awardTitleAdvanced tissue engineering strategies for biofabrication of complx tissues
oaire.awardTitleBioengineered Silk Fibroin-Based Contructs with Potential for Osteochondral Tissue Regeneration
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F99555%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F113803%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F113806%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/M-ERA-NET%2F0001%2F2014/PT
oaire.citation.endPage3799
oaire.citation.issue4
oaire.citation.startPage3781
oaire.citation.titleACS Applied Materials and Interfacespt_PT
oaire.citation.volume11
oaire.fundingStreamPOR_NORTE
oaire.fundingStreamOE
oaire.fundingStream3599-PPCDT
person.familyNameRibeiro
person.familyNamePina
person.familyNameCengiz
person.familyNameGarcía-Fernández
person.familyNameOliveira
person.familyNameReis
person.givenNameDr. Viviana
person.givenNameSandra
person.givenNameIbrahim Fatih
person.givenNameLuis
person.givenNameJoaquim Miguel
person.givenNameRui L.
person.identifier231614
person.identifier832165
person.identifier.ciencia-id9414-CDFB-4371
person.identifier.ciencia-idBB15-A8E4-8CB4
person.identifier.ciencia-id0B16-8EF6-862A
person.identifier.ciencia-id6F11-C02E-8B6C
person.identifier.orcid0000-0002-3679-0759
person.identifier.orcid0000-0002-4361-1253
person.identifier.orcid0000-0003-0886-632X
person.identifier.orcid0000-0002-4179-2556
person.identifier.orcid0000-0001-7052-8837
person.identifier.orcid0000-0002-4295-6129
person.identifier.ridA-3670-2019
person.identifier.ridH-8133-2013
person.identifier.ridJ-3360-2013
person.identifier.ridA-7629-2012
person.identifier.ridH-8636-2012
person.identifier.ridA-8938-2008
person.identifier.scopus-author-id56104780500
person.identifier.scopus-author-id35293476600
person.identifier.scopus-author-id35262249400
person.identifier.scopus-author-id57202066972
person.identifier.scopus-author-id56861715700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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