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Effects of soil sterilization and metal spiking in plant growth promoting rhizobacteria selection for phytotechnology purposes

2019Journal article Restricted access
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dc.contributor.authorMoreira, Helena
dc.contributor.authorPereira, Sofia I. A.
dc.contributor.authorMarques, Ana P. G. C.
dc.contributor.authorRangel, António O. S. S.
dc.contributor.authorCastro, Paula M. L.
dc.date.accessioned2018-10-16T09:59:10Z
dc.date.available2018-10-16T09:59:10Z
dc.date.issued2019
dc.description.abstractThe contamination of the soil with heavy metals (e.g. Zn) is a serious and crosscutting issue worldwide. Phytotechnologies can minimize the negative impact of this problem using plants and microorganisms in soil rehabilitation. However, the efficiency of proper plant-microbe combinations is usually assessed using spiked and/or sterilized soils, which do not mimic the conditions in situ, and therefore can lead to outcomes that will not be observed under field situations. This study aimed to quantify the effect of soil origin and sterilization on the performance of the two plant growth promoting rhizobacteria (PGPR), Ralstonia eutropha 1C2 and Chryseobacterium humi ECP37, for promoting the growth and metal accumulation of maize plants. A two-experiment approach was applied: the PGPR were inoculated in maize plants growing in (i) sterilized soils spiked with Zn (0, 100, 500 and 1000 mg Zn kg−1); and in (ii) a field-contaminated soil, under sterilized and non-sterilized conditions (599 mg Zn kg−1). Biomass and Zn accumulation in the root and shoot, and Zn bioavailability in soils were determined. Additionally, lipid peroxidation, activity of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) were assessed in the shoots of plants grown in the field-contaminated soil, as well as the composition of the rhizospheric bacterial community. Zn in the soils negatively affected maize growth, and its effect was strongest in the field-contaminated soil. Overall, PGPR attenuated the negative effects of Zn by improving plant growth, although less pronounced in non-sterilized soils. Sterilization significantly reduced soil Zn availability and affected its' accumulation in plant tissues. Bioinoculants performance was also different in sterilized soil, i.e., bacteria had no effect in the accumulation of Zn but tended to increase the biomass of maize plants. Despite the higher Zn accumulation in shoot tissues, lipid peroxidation was lower whereas antioxidant enzymes were enhanced in non-sterilized soils, suggesting that plant antioxidant system functioned properly. PGPR tended to decrease the diversity of the rhizospheric community. This study highlights that while inoculation with PGPR is effective in increasing Zn bioavailability in soil, accumulation in the plant and maize growth in Zn-contaminated soils, the extent of their effect can be different depending on whether the soil is field-contaminated or metal spiked, and on whether is sterilized prior contaminated. Consequently, the effect of bacterial inoculants assessed exclusively in metal spiked soil and/or sterilized soil may be overestimated, and potentially not transferable to field conditions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMoreira, H., Pereira, S. I. A., Marques, A. P. G. C., Rangel, A. O. S. S., & Castro, P. M. L. (2019). Effects of soil sterilization and metal spiking in plant growth promoting rhizobacteria selection for phytotechnology purposes. Geoderma, 334, 72–81. https://doi.org/10.1016/j.geoderma.2018.07.025pt_PT
dc.identifier.doi10.1016/j.geoderma.2018.07.025pt_PT
dc.identifier.eid85050791681
dc.identifier.eissn1872-6259
dc.identifier.issn0016-7061
dc.identifier.urihttp://hdl.handle.net/10400.14/25780
dc.identifier.wos000447116100010
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationSOE1/P5/E0189
dc.relationFormulation of new biofertilizers for crop production: contributions to sustainable agricultural practices
dc.relationAssessment of the energy production potential from phytoremediation derived biomass
dc.subjectSoil sterilizationpt_PT
dc.subjectPhytotechnologiespt_PT
dc.subjectZincpt_PT
dc.subjectPGPRpt_PT
dc.subjectOxidative stresspt_PT
dc.titleEffects of soil sterilization and metal spiking in plant growth promoting rhizobacteria selection for phytotechnology purposespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFormulation of new biofertilizers for crop production: contributions to sustainable agricultural practices
oaire.awardTitleAssessment of the energy production potential from phytoremediation derived biomass
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F105152%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F108359%2F2015/PT
oaire.citation.endPage81
oaire.citation.startPage72
oaire.citation.titleGeodermapt_PT
oaire.citation.volume334pt_PT
oaire.fundingStream5876
person.familyNamePereira
person.familyNameMarques
person.familyNameSantos Silva Rangel
person.familyNameCastro
person.givenNameSofia
person.givenNameAna
person.givenNameAntónio Osmaro
person.givenNamePaula
person.identifier2013444
person.identifier.ciencia-id3416-CFB7-17A6
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0002-5897-687X
person.identifier.orcid0000-0001-8232-1155
person.identifier.orcid0000-0002-6486-8947
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridH-4202-2016
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id15127794800
person.identifier.scopus-author-id14523081300
person.identifier.scopus-author-id7004827387
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery4638a49c-5d2a-4f86-a5c6-f89b3dad092b
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