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Authors
Gullón, Beatriz
Gullón, Patrícia
Tavaria, Freni
Pintado, Manuela
Gomes, Ana M. P.
Advisor(s)
Abstract(s)
Wheat bran (WB) samples were subjected to two stage processing (aqueous extraction and
hydrothermal treatment) to assess their potential as a raw material for the manufacture of
xylan-derived prebiotics. The liquid phase from the second stage, containing hemicellulose-
derived soluble arabinoxylooligosaccharides (AXOS), was refined by consecutive steps
of membrane filtration and ion exchange. The purified AXOS were characterised by matrixassisted
laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF-MS) and
chromatographic techniques. The most complex saccharide identified was made up of 19
pentoses. Human faecal slurry cultures were used to assess the bifidogenic activity of AXOS
and their effects on the production of Short Chain Fatty Acids (SCFA) and lactic acid. The
results were compared with data obtained using fructooligosaccharides (FOS), which are
accepted as the gold standard of a prebiotic ingredient. The stimulatory effects reached
by AXOS upon the bifidobacterial population were of the same order as those obtained with
FOS. Higher SCFA production was observed with AXOS in comparison with FOS.
Description
Keywords
Wheat bran Arabinoxylooligosaccharides Hydrothermal treatment Chemical structure Prebiotic Bifidobacteria
Citation
GULLÓN, Beatriz...[et al.] - Structural features and assessment of prebiotic activity of refined arabinoxylooligosaccharides from wheat bran. Journal of Functional Foods. ISSN 1756-4646. Vol. 6 ( 2014 ) , p. 438 –449
Publisher
Elsevier