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A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis

2023-09-06Journal article Open access
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dc.contributor.authorCarmo, Catarina
dc.contributor.authorCoelho, João
dc.contributor.authorSilva, Rui D.
dc.contributor.authorTavares, Alexandra
dc.contributor.authorBoavida, Ana
dc.contributor.authorGaetani, Paola
dc.contributor.authorGuilgur, Leonardo G.
dc.contributor.authorMartinho, Rui Gonçalo
dc.contributor.authorOliveira, Raquel A.
dc.date.accessioned2023-09-20T15:54:37Z
dc.date.available2023-09-20T15:54:37Z
dc.date.issued2023-09-06
dc.description.abstractMitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs after substantial chromosome compaction and is not promoted by condensin I. Instead, we show that the timely removal of transcripts from mitotic chromatin is driven by the SNF2 helicase-like protein Lodestar (Lds), identified here as a modulator of sister chromatid cohesion defects. In addition to the eviction of nascent transcripts, we uncover that Lds cooperates with Topoisomerase 2 to ensure efficient sister chromatid resolution and mitotic fidelity. We conclude that the removal of nascent transcripts upon mitotic entry is not a passive consequence of cell cycle progression and/or chromosome compaction but occurs via dedicated mechanisms with functional parallelisms to sister chromatid resolution.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.15252/embr.202256463pt_PT
dc.identifier.eid85165199957
dc.identifier.issn1469-221X
dc.identifier.pmcPMC10481674
dc.identifier.pmid37462213
dc.identifier.urihttp://hdl.handle.net/10400.14/42529
dc.identifier.wos001031927900001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCohesinpt_PT
dc.subjectCondensinpt_PT
dc.subjectMitotic transcriptionpt_PT
dc.subjectSNF2 familypt_PT
dc.subjectTopoisomerase 2pt_PT
dc.titleA dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.titleEMBO Reportspt_PT
oaire.citation.volume24pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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