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Influence of PDLA nanoparticles size on drug release and interaction with cells

2018Journal article Restricted access
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dc.contributor.authorCartaxo, Ana Luísa
dc.contributor.authorCosta-Pinto, Ana R.
dc.contributor.authorMartins, Albino
dc.contributor.authorFaria, Susana
dc.contributor.authorGonçalves, Virgínia M. F.
dc.contributor.authorTiritan, Maria Elizabeth
dc.contributor.authorFerreira, Helena
dc.contributor.authorNeves, Nuno M.
dc.date.accessioned2018-12-19T16:35:59Z
dc.date.available2018-12-19T16:35:59Z
dc.date.issued2018
dc.description.abstractPolymeric nanoparticles (NPs) are strong candidates for the development of systemic and targeted drug delivery applications. Their size is a determinant property since it defines the NP–cell interactions, drug loading capacity, and release kinetics. Herein, poly(D,L-lactic acid) (PDLA) NPs were produced by the nanoprecipitationmethod, in which the influence of type and concentration of surfactant as well as PDLA concentration were assessed. The adjustment of these parameters allowed the successful production of NPs with defined medium sizes, ranging from 80 to 460 nm. The surface charge of the different NPs populations was consistently negative. Prednisolone was effectively entrapped and released from NPs with statistically different medium sizes (i.e., 80 or 120 nm). Release profiles indicate that these systems were able to deliver appropriate amounts of drug with potential applicability in the treatment of inflammatory conditions. Both NPs populations were cytocompatible with human endothelial and fibroblastic cells, in the range of concentrations tested (0.187–0.784 mg/mL). However, confocal microscopy revealed that within the range of sizes tested in our experiments, NPs presenting amedium size of 120 nmwere able to be internalized in endothelial cells. In summary, this study demonstrates the optimization of the processing conditions to obtain PDLA NPs with narrow size ranges, and with promising performance for the treatment of inflammatory diseases.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCartaxo, A.L.. Costa-Pinto, A.R., Martins, A., Faria, S., Gonçalves, V.M.F., Tiritan, M.E., Ferreira, H., Neves, N.M. (2018). Influence of PDLA nanoparticles size on drug release and interaction with cells. Journal of Biomedical Materials Research part A, 107(3), 482-493pt_PT
dc.identifier.doi10.1002/jbm.a.36563pt_PT
dc.identifier.eid85057459610
dc.identifier.eissn1552-4965
dc.identifier.issn1549-3296
dc.identifier.pmid30485652
dc.identifier.urihttp://hdl.handle.net/10400.14/26475
dc.identifier.wos000459320900001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley & Sons
dc.relationRL2-SCN-NORTE-07-0124-FEDER-000018
dc.subjectPDLA nanoparticlespt_PT
dc.subjectSize distributionpt_PT
dc.subjectPrednisolonept_PT
dc.subjectCytocompatibilitypt_PT
dc.subjectCell internalizationpt_PT
dc.titleInfluence of PDLA nanoparticles size on drug release and interaction with cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F90332%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F38939%2F2007/PT
oaire.citation.endPage493
oaire.citation.issue3
oaire.citation.startPage482
oaire.citation.titleJournal of Biomedical Materials Research Part Apt_PT
oaire.citation.volume107
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
person.familyNameMartins
person.familyNameFaria
person.familyNameFerreira Gonçalves
person.familyNameTiritan
person.familyNameNeves
person.givenNameAlbino
person.givenNameSusana
person.givenNameVirgínia Maria
person.givenNameMaria Elizabeth
person.givenNameNuno
person.identifier1310409
person.identifier166319
person.identifier.ciencia-id341C-58AD-E483
person.identifier.ciencia-idAF19-FD90-5C40
person.identifier.ciencia-idDB12-D51F-C2D7
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.ciencia-id3013-6C92-AC79
person.identifier.orcid0000-0003-3868-0251
person.identifier.orcid0000-0001-8014-9902
person.identifier.orcid0000-0002-9151-516X
person.identifier.orcid0000-0003-3320-730X
person.identifier.orcid0000-0003-3041-0687
person.identifier.ridC-8432-2012
person.identifier.ridC-2176-2015
person.identifier.ridK-9492-2014
person.identifier.ridB-4685-2008
person.identifier.scopus-author-id10139137800
person.identifier.scopus-author-id36095519400
person.identifier.scopus-author-id23097687200
person.identifier.scopus-author-id6602890981
person.identifier.scopus-author-id7004622219
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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