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Development of a microfluidic paper-based analytical device for magnesium determination in saliva samples

dc.contributor.authorAguiar, Juliana I. S.
dc.contributor.authorSilva, Mafalda T. S.
dc.contributor.authorFerreira, Helena A. G.
dc.contributor.authorPinto, Elisabete C. B.
dc.contributor.authorVasconcelos, Marta W.
dc.contributor.authorRangel, António O. S. S.
dc.contributor.authorMesquita, Raquel B. R.
dc.date.accessioned2022-08-31T13:48:29Z
dc.date.available2022-08-31T13:48:29Z
dc.date.issued2022-12
dc.description.abstractIn this work, a microfluidic paper-based analytical device (μPAD) was developed for magnesium determination in saliva samples. The idea was to develop a fast and simple method for biological magnesium quantification targeting saliva as an easy to collect and non-invasive alternative to blood or urine samples. The μPAD approach was chosen due to its advantages, namely ideally suited to conduct on-location determinations, and not requiring trained operators or specialized laboratory equipment. The developed μPAD was based on the colorimetric reaction between eriochrome cyanine and magnesium to form an intense orange/reddish colour product. The colour intensity was determined by image processing after digital scanning, made within 10 to 90 min after sample loading. Under optimal conditions, the dynamic concentration range was 82–247 μM, with detection and quantification limits of 62 μM and 81 μM, respectively. The device is stable for up to 3 months when stored in vacuum or in a modified nitrogen atmosphere. An accuracy assessment was made by comparing the results obtained using the developed μPAD with those from atomic absorption spectrometry (AAS). The relative difference between the two sets of results was below 5%.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.talo.2022.100135pt_PT
dc.identifier.eid85135918757
dc.identifier.issn2666-8319
dc.identifier.urihttp://hdl.handle.net/10400.14/38646
dc.identifier.wos001007774500013
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectDisposable devicept_PT
dc.subjectMagnesium quantificationpt_PT
dc.subjectPoint-of-care analysispt_PT
dc.subjectSaliva samplespt_PT
dc.subjectSalivary magnesium correlation with serum magnesiumpt_PT
dc.titleDevelopment of a microfluidic paper-based analytical device for magnesium determination in saliva samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleTalanta Openpt_PT
oaire.citation.volume6pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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