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HPLC-DAD, ESI–MS/MS, and NMR of lycopene isolated from P. guajava L. and Its biotechnological applications

2018Journal article Restricted access
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dc.contributor.authorAmorim, Adriany G. N.
dc.contributor.authorSouza, Jessica M. T.
dc.contributor.authorSantos, Raimunda C.
dc.contributor.authorGullón, Beatriz
dc.contributor.authorOliveira, Ana
dc.contributor.authorSantos, Luiz F. A.
dc.contributor.authorVirgino, Adamor L. E.
dc.contributor.authorMafud, Ana C.
dc.contributor.authorPetrilli, Helena
dc.contributor.authorMascarenhas, Yvonne
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorPintado, M.M.E
dc.contributor.authorLeite, José R. S. A.
dc.date.accessioned2018-03-12T11:48:14Z
dc.date.available2018-03-12T11:48:14Z
dc.date.issued2018
dc.description.abstractPsidium guajava L. have been reported to be a rich source of antioxidant compounds. Its carotenoids have been highlighted by their high antioxidant capacity, which offers several benefits for human health. In this sense, lycopene isomers need to be identified. In this work, the comprehensive chemical characterization, by HPLC-DAD, MS/MS, and NMR, of lycopene isolated from P. guajava L., antioxidant and antimicrobial activity of lycopene extracts and isolated lycopene are evaluated. The FTIR results reported a structure with Z configuration, confirmed by UV-Vis, with λmax = 448, 473, and 505 nm for 5-Z lycopene. Furthermore, MS/MS positive ionization shows one fragment m/z 309 [M-227]+, relatively abundant for isolated lycopene. Experimental and Theoretical NMR studies revealed that guava may contain 5-Z lycopene because of the similarity found among the peaks. Lycopene extracts presented higher antioxidant activity than isolated lycopene, from both P. guajava L. and tomato, when measured by ABTS and ORAC (r2 = 0.9995 and r2 = 0.9992, respectively). In addition, lycopene extract shows antibacterial efficacy against E. coli, S. aureus, and L. innocua, presenting MBC values of 20 mg mL−1. These results suggest that lycopene extract have potential applicability for food, cosmetics, and pharmaceutical industry.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmorim, A. G. N., Souza, J. M. T., Santos, R. C., Gullón, B., Oliveira, A., Santos, L. F. A.,… Leite, R. S. A. (2018). HPLC-DAD, ESI–MS/MS, and NMR of Lycopene Isolated From P. guajava L. and Its Biotechnological Applications. European Journal of Lipid Science and Technololgy, 120(3), art. n.º 1700330pt_PT
dc.identifier.doi10.1002/ejlt.201700330pt_PT
dc.identifier.eid85040691689
dc.identifier.eissn1438-9312
dc.identifier.issn1438-7697
dc.identifier.urihttp://hdl.handle.net/10400.14/24155
dc.identifier.wos000426501600009
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley and Sonspt_PT
dc.relation2014/02282-6pt_PT
dc.relationPDSE/99999.004236/2014-09pt_PT
dc.relation165177/2014-4pt_PT
dc.relationPhD/SFRH/BD/75041/2010pt_PT
dc.titleHPLC-DAD, ESI–MS/MS, and NMR of lycopene isolated from P. guajava L. and Its biotechnological applicationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F79941%2F2011/PT
oaire.citation.issue3
oaire.citation.titleEuropean Journal of Lipid Science and Technololgypt_PT
oaire.citation.volume120
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameGullón Estévez
person.familyNameOliveira
person.familyNamePetrilli
person.familyNameMascarenhas
person.familyNameDelerue-Matos
person.familyNamePintado
person.familyNameLeite
person.givenNameBeatriz
person.givenNameAna
person.givenNameHelena
person.givenNameYvonne
person.givenNameCristina
person.givenNameMaria Manuela
person.givenNameJosé Roberto
person.identifier1415634
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person.identifier.ridJ-3404-2013
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person.identifier.ridL-6914-2015
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person.identifier.scopus-author-id6503889474
person.identifier.scopus-author-id55937245800
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person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id57210373661
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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