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Integrated liquid chromatography method in enantioselective studies: biodegradation of ofloxacin by an activated sludge consortium

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dc.contributor.authorMaia, Alexandra S.
dc.contributor.authorCastro, Paula M. L.
dc.contributor.authorTiritan, M. E.
dc.date.accessioned2017-10-13T18:46:18Z
dc.date.available2017-10-13T18:46:18Z
dc.date.issued2016
dc.description.abstracttOfloxacin is a chiral fluoroquinolone commercialized as racemate and as its enantiomerically pure formlevofloxacin. This work presents an integrated liquid chromatography (LC) method with fluorescencedetection (FD) and exact mass spectrometry (EMS) developed to assess the enantiomeric biodegradationof ofloxacin and levofloxacin in laboratory-scale microcosms. The optimized enantioseparation condi-tions were achieved using a macrocyclic antibiotic ristocetin A-bonded CSP (150 × 2.1 mm i.d.; particlesize 5 m) under reversed-phase elution mode. The method was validated using a mineral salts mediumas matrix and presented selectivity and linearity over a concentration range from 5 g L−1(quantificationlimit) to 350 g L−1for each enantiomer. The method was successfully applied to evaluate biodegrada-tion of ofloxacin enantiomers at 250 g L−1by an activated sludge inoculum. Ofloxacin (racemic mixture)and (S)-enantiomer (levofloxacin) were degraded up to 58 and 52%, respectively. An additional degrad-able carbon source, acetate, enhanced biodegradation up to 23%. (S)-enantiomer presented the highestextent of degradation (66.8%) when ofloxacin was supplied along with acetate. Results indicated slightlyhigher biodegradation extents for the (S)-enantiomer when supplementation was done with ofloxacin.Degradation occurred faster in the first 3 days and proceeded slowly until the end of the assays. Thechromatographic results from LC-FD suggested the formation of the (R)-enantiomer during levofloxacinbiodegradation which was confirmed by LC–MS with a LTQ Orbitrap XL.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMAIA, Alexandra S.; CASTRO, Paula M. L.; TIRITAN, M. E. - Integrated liquid chromatography method in enantioselective studies: Biodegradation of ofloxacin by an activated sludge consortium. Journal of Chromatography B. ISSN 1570-0232. Vol. 1029 (2016), p. 174-183pt_PT
dc.identifier.doi10.1016/j.jchromb.2016.06.026pt_PT
dc.identifier.eid84978194444
dc.identifier.pmid27433982
dc.identifier.urihttp://hdl.handle.net/10400.14/23087
dc.identifier.wos000381651900023
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationFCOMP-01-0124-FEDER-022718pt_PT
dc.relationFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
dc.relationFLUOROQUINOLONE ANTIBIOTICS IN THE ENVIRONMENT: BIODEGRADATION STUDIES AND PRESENCE IN SEVERAL ENVIRONMENTAL MATRICES.
dc.relationStrategic Project - LA 16 - 2011-2012
dc.relation2014 - Strategic Project
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.subjectOfloxacinpt_PT
dc.subjectLevofloxacinpt_PT
dc.subjectMacrocyclic antibiotic-based stationary phasespt_PT
dc.subjectEnantioselectivitypt_PT
dc.subjectBiodegradationpt_PT
dc.subjectActivated sludgeapt_PT
dc.titleIntegrated liquid chromatography method in enantioselective studies: biodegradation of ofloxacin by an activated sludge consortiumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
oaire.awardTitleFLUOROQUINOLONE ANTIBIOTICS IN THE ENVIRONMENT: BIODEGRADATION STUDIES AND PRESENCE IN SEVERAL ENVIRONMENTAL MATRICES.
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardTitle2014 - Strategic Project
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-EBI%2F111699%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F86939%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FSAU%2FUI4040%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04423%2F2013/PT
oaire.citation.endPage183
oaire.citation.startPage174
oaire.citation.titleJournal of Chromatography Bpt_PT
oaire.citation.volume1029
oaire.fundingStream3599-PPCDT
oaire.fundingStreamFARH
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMaia
person.familyNameCastro
person.familyNameTiritan
person.givenNameAlexandra S.
person.givenNamePaula
person.givenNameMaria Elizabeth
person.identifier2013444
person.identifier1310409
person.identifier.ciencia-id6A15-F0A3-AEF2
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.orcid0000-0002-4167-383X
person.identifier.orcid0000-0001-8841-6606
person.identifier.orcid0000-0003-3320-730X
person.identifier.ridM-8241-2013
person.identifier.ridK-9492-2014
person.identifier.scopus-author-id55332349300
person.identifier.scopus-author-id7102781782
person.identifier.scopus-author-id6602890981
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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