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Autolysis of cell walls from polygalacturonase-antisense tomato fruit in simulated apoplastic solutions

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dc.contributor.authorAlmeida, Domingos P. F.
dc.contributor.authorHuber, Donald J.
dc.date.accessioned2012-01-26T17:19:28Z
dc.date.available2012-01-26T17:19:28Z
dc.date.issued2011
dc.description.abstractAutolysis of cell walls from polygalacturonase (PG)-antisense tomato fruit was studied in a conventional buffer designed to maximize the catalytic activity of PG (30 mM sodium acetate, 150 mM NaCl, pH 4.5), and in solutions mimicking the pH and mineral composition of the fruit apoplast at the mature-green and ripe stages. Autolytic release of uronic acids was very limited under simulated apoplastic conditions compared with the conventional buffer, but minimal differences in the release of reducing groups were observed among the incubation conditions. Autolytic release of uronic acids from active walls was lower than solubilization from enzymically inactive walls. Uronic acids that remained ionically bound to the cell walls during autolysis were subsequently extracted and analyzed by size exclusion chromatography. The elution profiles of ionically bound uronic acids from cell walls incubated under optimal conditions were similar for all ripening stages. In solutions mimicking the pH and mineral composition of the apoplast of mature-green and ripe fruit, uronic acids extracted from pink and ripe fruit cell walls showed a decrease in average molecular mass compared with polymers from mature-green cell walls. The results suggest that the composition of the incubation solution exert strong influence on PG-independent cell wall autolysis and that enzymically active walls restrain PG-independent pectin solubilization.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationALMEIDA, Domingos P.F; HUBER, Donald J. - Autolysis of cell walls from polygalacturonase-antisense tomato fruit in simulated apoplastic solutions. Plant Physiology and Biochemistry. ISSN 0981-9428. Vol. 49, n.º 6 (2011), p. 617-622por
dc.identifier.doi10.1016/j.plaphy.2011.02.021
dc.identifier.issn0981-9428
dc.identifier.urihttp://hdl.handle.net/10400.14/7642
dc.identifier.wosWOS:000291417900008
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectCell wallpor
dc.subjectFruit ripeningpor
dc.subjectPectinpor
dc.subjectSolanum lycopersicumpor
dc.subjectUronic acidpor
dc.titleAutolysis of cell walls from polygalacturonase-antisense tomato fruit in simulated apoplastic solutionspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage622
oaire.citation.issue6
oaire.citation.startPage617
oaire.citation.titlePlant Physiology and Biochemistry
oaire.citation.volume49
person.familyNameAlmeida
person.givenNameDomingos
person.identifier215276
person.identifier.orcid0000-0001-8429-8585
person.identifier.ridF-1835-2010
person.identifier.scopus-author-id14827787500
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication27344dc4-31d7-49c7-90b5-28f0c5596130
relation.isAuthorOfPublication.latestForDiscovery27344dc4-31d7-49c7-90b5-28f0c5596130

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