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Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods

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dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorEgas, Conceição
dc.contributor.authorNunes, Olga C.
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2014-05-08T14:08:31Z
dc.date.available2014-05-08T14:08:31Z
dc.date.issued2013
dc.description.abstractThis study aimed to assess the influence of water treatment and distribution on the bacterial communities with particular emphasis on tap water. Samples from the water treatment plant, the bulk supply distribution system and household taps, supplied by the same drinking water treatment plant, were analyzed using culture-dependent and culture-independent methods. Water treatment imposed alterations in the composition of the bacterial community, although this effect was more evident in the cultivable bacteria rather than among the total community assessed by 16S rRNA gene-denaturing gradient gel electrophoresis (DGGE) profiling. Water disinfection, mainly chlorination, promoted a reduction on bacterial diversity and cultivability, with a shift in the pattern of cultivable bacteria from predominantly Gram-negative to predominately Gram-positive and acid-fast. Downstream of the chlorination stages, tap water, in comparison with raw water, presented higher diversity indices and cultivability percentages. From the source to the tap, members of the Alpha-, Beta- and Gammaproteobacteria were the predominant lineages identified using 16S rRNA gene-DGGE analysis. Although with a lower coverage, the DGGE-based lineage identifications were in agreement with those found using 454-pyrosequencing analysis. Despite the effectiveness of water treatment to eliminate or inactivate most of the bacteria, Proteobacteria such as Acinetobacter, Bosea and Sphingomonadaceae may successfully colonize tap water.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationVAZ-MOREIRA, Ivone ...[et al.] - Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods. FEMS Microbiology Ecology. ISSN 1574-6941. Vol. 83 (2013), p. 361–374por
dc.identifier.doi10.1111/1574-6941.12002
dc.identifier.eissn1574-6941
dc.identifier.issn0168-6496
dc.identifier.urihttp://hdl.handle.net/10400.14/14320
dc.identifier.wosWOS:000313252600009
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherOxford University Presspor
dc.relationStrategic Project - LA 16 - 2011-2012
dc.subjectBacterial diversitypor
dc.subjectCultivable bacteriapor
dc.subjectUncultivable bacteriapor
dc.subjectDrinking waterpor
dc.titleBacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methodspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAMB%2F70825%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F27978%2F2006/PT
oaire.citation.endPage374
oaire.citation.issue2
oaire.citation.startPage361
oaire.citation.titleFEMS Microbiology Ecology
oaire.citation.volume83
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameVaz-Moreira
person.familyNameEgas
person.familyNameNunes
person.familyNameManaia
person.givenNameIvone
person.givenNameConceição
person.givenNameOlga
person.givenNameCélia
person.identifier602434
person.identifier.ciencia-id9E17-544E-800D
person.identifier.ciencia-idBA14-BD30-C9AF
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0001-8912-9836
person.identifier.orcid0000-0002-2307-5414
person.identifier.orcid0000-0003-4742-2537
person.identifier.orcid0000-0002-3674-1789
person.identifier.ridK-4866-2014
person.identifier.scopus-author-id16044425800
person.identifier.scopus-author-id7005088596
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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